摘要
目的 应用现代化代谢组学前沿技术探索中药沙棘抗衰老的作用机制,为筛选延缓衰老的药物提供实验依据.方法 将SD大鼠分为4月龄组（n=10）、24月龄组（n=11）和沙棘干预24月龄组（n=12）.收集各组大鼠尿液进行1H NMR分析.应用Hotelling＆#39;s T2和趋势过滤方法分析各组尿液中代谢物,观察中药沙棘对于代谢物的影响.结果 鉴定13个与衰老有关的代谢物,中药沙棘干预组代谢谱发生明显改变.结论 中药沙棘可通过改善部分代谢途径延缓衰老,为抗衰老药物的筛选提供了新的实验方法和理论依据.
Objective To explore the anti-aging mechanism of Hippophae rhamnoides L using metabonomics in order to provide experimental data for drug development on anti-aging. Methods SD rats were divided into three groups： 4 months group, 24 months group, Hippophae rhamn0ides L treated group （24 months）. Urine of each rat was collected for metabolite analysis by 1H NMR based on the method from Hotelling＇ s T2 and method of trend filtering algorithm. Results The data showed that Hippophae rhamnoides L changed the metabolism spectrum of rat by identifying and analyzing 13 metabolite related to aging. Conclusion Hippophae rhamnoides L can delay the process of aging by changing the metabolism, which may provide a new theory and way for drug development against aging.
出处
《老年医学与保健》
CAS
2014年第3期169-172,共4页
Geriatrics & Health Care
基金
基金项目：上海市卫生局课题（20124090）
关键词
衰老
代谢组学
沙棘
尿液
Aging
Metabonomics
Hippophae rhamnoides L
Urine
