摘要
测定了15种植物叶片的甜菜碱含量。结果表明,抗盐碱、干旱和抗寒的植物含量较高。从菠菜叶片分离和部分纯化了甜菜碱醛脱氢酶,并对其某些性质进行了研究。甜菜碱醛脱氢酶存在于60%的硫酸铵部分,70%硫酸铵部分未检出其活性。该酶有两个同工酶,在-80℃条件下十分稳定,其活性为0.125—1.0mol/L的NaCl和KCl所抑制,但Mn^(2+)和Mo^(6+)离子对活性没有影响,而Mg^(2+)稍增加其活性。
Betaine content in leaves of fifteen plant species was determined.The results showed higher betaine levels in those salt-,drought-,and chilling-resistant species.Betaine aldehyde dehydrogenase (BADH,EC 1.2.1.8) was isolated and partially purified from spinach leaves. Some properties of this enzyme were studied.BADH was precipitated by 60% saturation of (NH_4)_2SO_4.Its activity was not detected in 70% saturation of (NH_4)_2SO_4.BADH has two isoenzymes.The activity of BADH was quite stable below-80℃.It was inhibited by 0.125- 1.0 mol/L NaCl or KCl,but not by Mn^(2+) and Mo^(6+),and slightly increased by Mg^(2+).
基金
国家自然科学基金
国家科技攻关计划资助课题
关键词
菠菜
甜菜碱醛
脱氢酶
氧化
Betaine aldehyde dehydrogenase
Spinach
Isoenzyme
Cation
