摘要
在干旱胁迫后检测了转BnDREB1-5基因烟草碳氮代谢指标和渗透调节物质的变化。结果表明,干旱胁迫后转基因烟草碳代谢指标淀粉酶活性、转化酶活性和可溶性糖含量都明显升高,野生型烟草淀粉酶活性和可溶性糖含量稍微升高,转化酶活性反而降低;转基因烟草氮代谢指标硝酸还原酶活性、可溶性蛋白质含量稍微下降,而野生型烟草两者都明显降低;转基因烟叶中有BADH活性,干旱胁迫后BADH活性升高,而野生型烟草中检测不到BADH活性;干旱胁迫后转基因烟叶中脯氨酸含量是野生型的1.08倍。因此,干旱胁迫后转基因烟草碳氮代谢、防御酶活性等方面明显优于野生型。
After drought stress the changes of the carbon and nitrogen metabolism and osmotic adjustment on tobacco with 35S： BnDREB1-5 transformation were analyzed.The results indicated that after physical drought-stress the carbon metabolisms of transgenic tobacco,which included the amylase activity,the invertase activity and the soluble sugar content,increased significantly,and the amylase activity and the soluble sugar content of wild-type increased slightly,but the invertase activity of wild-type decreased.The nitrogen metabolisms of transgenic tobacco,which included the nitrate reductase activity and the soluble protein content,decreased slightly,while those of wild-type tobacco decreased significantly.The BADH activity of transgenic tobacco leaves was detectable and increased after drought stress,but that of wild-type was not detectable.After drought stress,the proline content of transgenic tobacco leaves was 1.08 times as large as that of wild-type.After drought stress the carbon and nitrogen metabolism and defense activity on tobacco with 35S： BnDREB1-5 transformation were obviously better than wild-type.
出处
《中国烟草科学》
CSCD
北大核心
2011年第4期36-40,共5页
Chinese Tobacco Science
基金
河南省攻关项目(0624050012)
关键词
烟草
DREB转录因子
干旱胁迫
碳氮代谢
渗透调节
tobacco
DREB transcription factor
drought stress
carbon and nitrogen metabolism
osmotic adjustment
