摘要
目的研究葡萄球菌肠毒素A(SEA)对原发性肝细胞癌肿瘤浸润淋巴细胞 (TIL)和肝门淋巴结淋巴细胞抗癌活性的诱导作用。方法取 5例手术切除原发性肝细胞癌标本及肝门淋巴结1枚 ,分离TIL和淋巴细胞 ,在SEA作用下进行培养。定时记数 ,了解其增殖情况。流式细胞仪检测其CD3、CD4、CD8表达情况 ,MTT微量比色法测定其对HepG 2肝癌细胞株的细胞毒活性 ,ELISA法测定培养上清液中TNF α、IFN γ浓度。结果在SEA刺激下 ,2周时两组细胞均扩增 10 0倍。 1周后CD3+ 细胞占 95 %以上 ,CD8+ 细胞较CD4 + 细胞增殖更迅速。细胞毒活性随培养逐渐增强。在培养的前 10d内 ,产生大量的TNF α和IFN γ。结论SEA可高效、迅速诱导原发性肝细胞癌TIL和肝门淋巴结淋巴细胞的抗癌活性。
ObjectiveTo evaluate the antitumor effects of lymphocytes from hepatic porta lymph node co cultured with staphylococcal enterotoxin A(SEA)?MethodsLymphocytes isolated from lymph node of 5 HCC patients were co cultured with SEA. The proliferation and cytotoxicity of the lymphocytes were observed. Flow cytometer was used to detect the expression of CD3?CD4 and CD8 on the surface of the lymphocytes. TNF α and IFN γ released into the culture medium were measured by the method of ELISA. The results were compared with that yielded from TILs of the individual patients. ResultsCultured in the presence of SEA, there was a sharp rise of T cells, especially CD8 + cells. Cytotoxic activity was increased. TNF α and IFN γ were predominantly produced in the first week.ConclusionsLymphocytes from lymph node activated by SEA were armed with an increased antitumor efficacy.
出处
《中华普通外科杂志》
CSCD
北大核心
2002年第7期415-417,共3页
Chinese Journal of General Surgery
关键词
肠毒素类
淋巴细胞
淋巴结
肝细胞癌
Enterotoxins
Lymphocytes
Lymph nodes
Carcinoma, hepatocellular
