摘要
在肠卷石蜡法切片上 ,用ABC免疫组织化学方法 ,显示消化管全长的生长抑素分泌细胞 (又称D细胞 ) ,结果只在胃体部显现 ,主要散在分布于胃腺中 ,个别出现在粘膜上皮。消化道内分泌细胞根据基部有否胞质突起 ,可分为 4种类型 ,在黄鳝胃中均有 ,其中Ⅲ型数量最多 ,Ⅰ、Ⅳ型次之 ,而Ⅱ型数量最少。由于产生生长抑素的D细胞在消化道内分泌细胞中作用多并且类型复杂 ,故黄鳝胃还是一个十分重要且复杂的内分泌器官。应用透射电镜 ,对黄鳝的胃肠作超薄切片观察显示在胃腺上皮细胞内含大量的微管泡系和一定量的酶原颗粒 ,表明其兼有泌酸及产生酶原的功能 ,属泌酸胃酶细胞。在肠上皮细胞游离面 ,含有大量微绒毛 ,但幼鳝比成鳝的长、密且整齐 ,提示幼鳝的肠上皮比成鳝有更强的吸收能力。
Somatostatin secreting cells (named D cells also) in whole digestive tract of mud eel were showed with ABC immunohistochemical method in cured gut paraffin section. The somatostatin secreting cells were only presented in gastric body, scattered mainly in gastric gland and a few were showed in mucous epithelium. According to cytoplasmic process of their bottom , four endocrine cell types could be distinguished in digestive tract and they all were presented in stomach of mud eel, but the number of type Ⅲ was the largest, typesⅠand Ⅳ were next, type Ⅱwas the smallest. D cells producing somatostatin had the most functions and the most complex types among endocrine cells in digestive tract, so stomach of mud eel was also an important endocrine organ. Epithelial cell of gastric gland observed with electron microscopy contained lots of tubuloresicular systems and some zymogen granules, which showed it could secrete acid and produce zymogen, so it belonged to oxynticopeptic cell. Apical membrane of intestinal epithelial cell contained lots of microrillis. Microrillis of juvenile mud eel were longer, denser and tidier that those of adult mud eel, which showed absorbability of juvenile mud eel was stronger than absorbability of adult mud eel.
出处
《水产学报》
CAS
CSCD
北大核心
2002年第3期202-216,共15页
Journal of Fisheries of China
基金
浙江省科技厅重点科研资助项目 ( 98110 2 0 39)
杭州市科委资助项目 ( 9812 1B0 9)
关键词
黄鳝
胃肠
生长抑素分泌细胞
超微结构
免疫组织化学
Monopterus albus
stomach and intestine
somatostatin secreting cells
immunohistochemistry
ultrastructure
