摘要
本文以自然授粉的‘春蕾’、‘沪017’、‘布目早生’、‘1-4-8’、‘7-6-9’的胚珠为试材,研究了早熟和特早熟桃胚珠培养胚成苗的有关影响因素。本试验5个品种(品系)的结果表明,桃品种或品系的果实生育期愈短,胚珠培养胚的成苗率愈高,特早熟桃‘春蕾’花后48天的胚珠培养在改良S H培养基附加BA1.0mg/1+IAA1.0mg/1上30天,将长大的幼胚转移至TM培养基上,胚的成苗率达75％。此外,作者对胚珠培养技术在桃育种中的应用范围及其培养程序开展了讨论。
This paper deals with some factors affecting plantlet formation from in-ovulo embryo culture of early-maturing and very early-maturing peaches with the open-pollinsted ovules of 'Chun Lei', 'Hu 017% 'Nu-nome Wase', '1-48', '7-6-9' as experimental materials.
The results were as follows.The shorter the fruit developing period of the varieties or strains was, the higher the plant formation rates of the young embryos after in-ovulo embryo culture, When the ovules of 'Chun Lei', 48 days after anthesis, were cultured on the modified SH medium with the addition of BA l.0mg/1 + IAA l.0mg/1 for 1 month, the in-ovulo embryos developed best. When the grown young embryos were transferred to TM medium, the percent of plant formation could reach 75%.
The scope of in-ovulo embryo culture applied in peach breeding and the procedure of in-ovulo embryo culture were discussed.
出处
《园艺学报》
CAS
CSCD
北大核心
1991年第4期303-308,共6页
Acta Horticulturae Sinica
关键词
早熟
胚珠培养
植株再生
桃
育种
Early-maturing peach
Very early-maturing peach
Inovulo embryo culture
Plantlet formation
