摘要
目的 研究褪黑素对小鼠免疫性肝损伤的保护作用。方法 在诱导BCG +LPS免疫性肝损伤模型的基础上 ,用分光光度法检测血浆中ALT、AST、NO水平和肝匀浆MDA、SOD含量 ;L92 9细胞株法检测血浆中TNF α的生物学活性 ;胸腺细胞增殖法测定血浆中IL 1活性。结果 在BCG+LPS诱导的免疫性肝损伤中 ,褪黑素 (0 2 5 ,1,4mg·kg-1)ig预防给药均明显降低免疫性肝损伤小鼠增高的血浆ALT、AST活性 ,且以 1mg·kg-1的剂量作用最明显 ;并能减少肝匀浆MDA含量 ,使降低的肝匀浆SOD活性升高 ,抑制免疫性肝损伤小鼠血浆NO、TNF α和IL 1的升高。
AIM To investigate the prophylactic effect of melatonin (MT) on immunological liver injury. METHODS The model of mouse immunological liver injury was induced by injection of bacillus calmette guerin (BCG) and lipopolysaccharide (LPS) from tail vein. The alanine aminotransferase(ALT), aspartate aminotransferase(AST), nitric oxide (NO) level in plasma, malondiadehyde(MDA) content and superoxide dismutase(SOD) activity in liver homogenate were assayed with different methods; Tumour necrosis factor α (TNF α) activity was determined by method of L929 cells line; Interleukin 1 activity was determined by method of thymocytes proliferation. RESULTS Immunological liver injury was successfully induced by BCG+LPS. MT(0 25,1,4 mg·kg -1 ) was found to decrease significantly the serum transaminase (ALT,AST) activities.However,MT at the dose of 1 mg·kg -1 was the most effective among the three doses. MT also decreased MDA content and improved reduced SOD level in liver homogenate. Furthermore, MT significantly reduced TNF α, NO and IL 1 levels in serum. CONCLUSION MT shows significant protective action on immunological liver injury in mice.
出处
《中国药理学通报》
CAS
CSCD
北大核心
2002年第3期331-333,共3页
Chinese Pharmacological Bulletin
