摘要
目的:探讨褪黑素(MT)对实验性慢性乙醇肝损伤小鼠脂质过氧化、肝功能和肝脏病理组织的影响.方法:将48只小鼠随机分为4组:对照组(A组),模型组(B组),造模+高浓度MT给药组(C组),造模+低浓度MT给药组(D组).用60 mL/L乙醇制成小鼠慢性肝损伤模型,测定肝组织中丙二醛(MDA)含量、天冬氨酸转氨酶(AST)和丙氨酸转氨酶(ALT)的活性,切片观察肝脏病理损害程度并评分.结果:MT使慢性乙醇慢性肝损伤小鼠的MDA含量明显下降,有效降低AST和ALT活性,并与剂量呈正相关.MDA:造模+高浓度MT给药组和造模+低浓度MT给药组值均明显较模型组减低(P均<0.05).ALT:造模+低浓度MT给药组活性值明显低于模型组(P<0.01);造模+高浓度MT给药组活性值明显较造模+低浓度MT给药组减低(P<0.01).AST:造模+低浓度MT给药组活性值明显低于模型组(P<0.01);造模+高浓度MT给药组活性值明显较造模+低浓度MT给药组减低(P<0.01).病理学形态观察显示,MT能减轻肝脏病理损害程度,与剂量成正相关.病理损害程度评分:造模+低浓度MT给药组值明显低于模型组(P<0.01);造模+高浓度MT给药组值明显较造模+低浓度MT给药组低(P<0.01).结论:MT对慢性乙醇肝损伤具有一定的保护作用,且与药物剂量呈正相关.
AIM： To investigate the changes of alanine aminotransferase （ ALT ）, aspartate aminotransferase （ AST ）, malondialdehyde （MDA） and pathological lesions of mice livers, which were caused by chronic alcohol drinking, and the protective effect of melatonin（MT） against ethanol-induced chronic hepatotoxicity. METHODS： Forty-eight mice were randomly divided into 4 groups： control group（A）, alcohol group （B）, alcohol ＋ high dosage MT group （ C ） , and alcohol ＋ low dosage MT group （D）. 60 mL/L alcohol was used to induce hepatotoxicity in these mice. MT was injected at 20 and 5 mg/kg immediately after alcohol drinking in group C and group D respectively. The activities of ALT, AST and the levels of MDA in liver homogenate were detected. And pathological lesion in liver tissue was evaluated. RESULTS： MT inhibited the increasing tread of MDA in liver homogenate and lowered the levels of ALT and AST in chronically ethanol-treated mice. MDA： the content in group D and C was lower than that of group B （P〈0.05）; ALT： the activity in group D was lower than that of group B （P〈0.01） , and the activity in group C was lower than that of group D （P 〈 0.01） ; AST： the activity in group D was lower than that of group B （P 〈 0.01 ） ; the activity in group C was lower than that of group D （ P 〈0.01 ）. And in morphology MT could greatly prevent the lesions of chronically ethanol-treated mice livers. Total pathological score： the score of group D was much lower than that of group B （P 〈0.01 ） ; the score of group C was greatly lower than that of group D （P 〈0.01 ）. CONCLUSION： MT indeed hasa protective effect on chronic ethanol-induced mice livers, which is positively associated with MT dosage.
出处
《第四军医大学学报》
北大核心
2006年第18期1665-1667,共3页
Journal of the Fourth Military Medical University
关键词
褪黑激素
乙醇
肝/损伤
慢性病
melatonin, ethanol liver/injuries
chronic disease
