摘要
由于抗生素及消毒设施的使用 ,致使许多细菌转化为L型菌 ,增加了医院感染的隐患。为了解医院环境的L型菌污染情况 ,在日常监测的基础上增加L型菌培养 ,同时采用紫外线及含氯消毒剂诱导L型大肠杆菌、绿脓杆菌及枯草杆菌。结果 :L型菌普遍存在于物体表面、空气及消毒液中 ,碘伏棉球中未见L型菌 ,酒精棉球中 60 %为有菌生长 ;次氯酸钠浓度 <5∶10 0 0 0 ,作用时间 <3 0min时 ,易诱导出L型菌 ,次氯酸钠浓度 >5∶10 0 0 0时 ,未诱导出L型菌 ;采用紫外线照射 60min时 ,可诱导出L型菌。说明增加L型菌培养 ,可明显增加细菌检出率。
As a result of the use of antibiotics and disinfection facilities, many bacteria have changed to be L-bacteria. In this case, it increases the risk of iatrogenic infection. To understand the L-bacteria contamination status of hospital environment, culture of L-bacteria was performed on the basis of routine monitoring. Meanwhile, ultraviolet ray and chlorinated disinfectant were adopted to induce Escherichia coli, Pseudomonas aeruginosa, and hay bacillus to become L-bacteria. Result showed that L-bacteria were commonly existed on the surface of objects. There were no L-bacteria either in air, disinfectant solution, or in iodophor cotton balls. Bacterial growth can be seen in 60% of alcohol cotton balls. While the concentration of sodium hypochlorite was < 5∶10000 and action time was less than 30 min, L-bacteria can easily be induced. However, while sodium hypochlorite concentration was >5∶10000, L-bacteria can not be induced. After ultraviolet ray irradiation for 60 min, L-bacteria can also be induced. Suggested that by adding L-bacteria culture, it can enhance the bacterial detectable rate.
出处
《护理研究》
2002年第7期397-399,共3页
Chinese Nursing Research
关键词
医院环境
L型菌
细菌培养
次氯酸钠
紫外线照射
Bacterial culture
Hospital environment
L-bacteria
Sodium hypochlorite
Ultraviolet ray irradiation
