摘要
[目的 ]检测第三军医大学附属西南医院ICU临床分离黄杆菌产 β -内酰胺酶 (bla)、超广谱 β -内酰胺酶(ESBLs)情况及对临床常用抗生素的敏感性 ,以期为治疗黄杆菌属感染的经验用药方案和预防感染护理措施的制订提供实验依据。 [方法 ]用纸片法、PCR扩增法检测产bla情况 ;双纸片协同法检测产ESBLs情况 ;细菌对抗生素的敏感性用K -B法和梯度琼脂平板法测定。 [结果 ] 3株黄杆菌均产bla和ESBLs ,且对 β -内酰胺类抗生素耐药率高 ,对氟喹诺酮类抗生素及加酶头孢菌素较敏感。 [结论 ]院内感染黄杆菌普遍产bla ,对 β -内酰胺类抗生素耐药率高 ,可优先选用氟喹诺酮类抗生素或加酶抑制剂的 β -内酰胺类抗生素治疗该菌导致的感染 。
Objective: to determine both β-Lactamase and extended-spectrum β-Lactamase (ESBLs) produced by Flavobacterium isolated from ICU in affiliated southwest hospital of the third military medical university, and the sensitivity to antibiotics commonly used in clinic, in order to providing an experimental basis for working out experiential medical scheme and preventive nursing methods for Flavobacterium infection. Methods: the state of β-Lactamase-producing was measured by paper strip method and PCR amplified method. The extended-spectrum β-Lactamase producing state was determined by double paper strip synergistic method. And sensitivity of the bacterium to antibiotics was measured by using gradient agar diffusion of K-B methods. Results: 3 strains of Flavobacteria were producing bothβ-lactamases and ESBLs. So it had a high drug-resistance to antibiotics. However, it is sensitive to both fluoroquinolone and cephalosporin with β-lactamase. Conclusions: Flavobacterium of iatrogenic infection generally produce β-lactamase and ESBLs. It has a high drug resistance for β-lactam antibiotics. Fluoroquinolone or antibiotics with blocking agent can be chosen in advance in curing the infection caused by Flavobacterium. One should strengthen nursing measures and control the transfer of resistance plasmids.
基金
"十五"军队重点科研基金课题 (课题号 :No .0 1L0 65)
关键词
黄杆菌
超广谱Β-内酰胺酶
抗生素
ICU
Flavobacterium
β-Lactamase
extended-spectrum β-Lactamase (ESBLs)
antibiotics
ICU
