摘要
目的 :探讨血管内皮生长因子 (VEGF)、p5 3蛋白的表达与乳腺癌血管新生及浸润、转移的关系。 方法 :应用免疫组织化学S P法检测VEGF、p5 3在 76例乳腺癌组织和 2 0例良性乳腺病变组织中的表达 ,并应用计算机辅助图像分析系统对血管新生的强度进行定量分析 ,评估VEGF、p5 3表达以及微血管计数 (MVC)与浸润和转移的关系。结果 :癌组织VEGF和 p5 3表达阳性分别为 6 1例 (80 3% )和 4 5例 (5 3 9% ) ,表达强度均明显高于良性病变 (P <0 0 0 5 ) ,VEGF的表达强度在原位癌和浸润癌间差异有极显著性意义 (P <0 0 0 1) ,但是与淋巴结转移与否无明显关联 (P =0 39) ,p5 3的表达强度与浸润或转移均无明显关联 (P >0 1)。良性病变组织、导管原位癌及浸润性导管癌的MVC差异有显著性 (P <0 0 5 ) ;癌组织的MVC平均为12 8 6± 4 3 7,且随着病变进展有明显增加的趋势。VEGF表达强度与MVC显著关联 (P =0 0 0 7) ,而 p5 3表达强度则与MVC无关 (P =0 34)。浸润性导管癌无论淋巴结是否转移 ,其VEGF、p5 3表达及MVC均无明显差异。结论 :VEGF与癌组织的血管新生及浸润生长关系密切 ,联合VEGF表达和MVC检测可提供预示乳腺癌恶性转归和预后更有价值的指标。
Purpose To determine the correlation of vascular endothelial growth factor(VEGF) and p53 protein with tumor neovascularization and metastasis in breast carcinoma. Methods The expression of VEGF, p53 protein and FⅧRAg in 76 breast carcinomas and 20 benign breast lesions were studied with S P immunohistochemical technique, the extent of neovascularization was assessed quantitatively by computer assisted video analysis system. Microvessel count (MVC) and the expressions of VEGF and p53 protein were estimated in different invasive and metastatic status of breast carcinoma. Results 61 cases for VEGF (80 3%) and 45 cases for p53 protein (53 9%) were positive and the average MVC was 128 6±43 7 in the breast carcinoma group. Significant differences between the MVC and the expression of VEGF were observed among the benign lesions, ductal carcinoma in situ (DCIS) and invasive ductal carcinoma ( P< 0 05). It showed that the tissue's MVC tended to increase with the malignant progression of tumors. The expression of VEGF and p53 protein in carcinoma group was significant higher than benign group ( P< 0 05). VEGF levels had significant difference between DCIS and invasive carcinoma ( P< 0 001), and were found a significant association with MVC ( P =0 007), but not related to lymph node status ( P =0 39). p53 protein levels were found not related to invasion, metastasis ( P> 0 1) and MVC ( P =0 34). MVC, VEGF and p53 protein had no significant difference between metastasis and non metastasis group in invasive ductal carcinoma. Conclusions It is suggested that VEGF takes an important role in tumor neovascularization. Combining examination of VEGF with MVC will be a better predictor for malignant degree and prognosis in breast carcinoma.
出处
《临床与实验病理学杂志》
CAS
CSCD
2002年第2期185-188,共4页
Chinese Journal of Clinical and Experimental Pathology
基金
广东省卫生厅基金 (A19982 88)
国务院侨办基金(93A13 0 )资助项目
