摘要
用相对定量RT PCR的方法研究二花脸猪 (Erhualian)和大白猪 (1argeWhite)胃组织中生长激素受体 (GHr)mR NA发育性变化并进行品种间比较。分别于出生当天、3、2 0、30、4 5、90、12 0和 180日龄随机选取纯种大白猪公猪和二花脸公猪各 4头宰杀并采样 ,同时记录胃重和体重。研究结果表明 :出生时胃组织中GHrmRNA水平较高 ,3日龄时显著下调 (P <0 0 5 ) ;二花脸猪胃组织中GHrmRNA水平在断奶时 (45日龄 )达到高峰 ,随后显著下降 (P <0 0 5 ) ,至 90日龄后维持相对稳定。大白猪公猪在 90日龄时胃组织中GHrmRNA水平达到高峰 ,并维持在较高水平 ;从出生到断奶 ,二花脸猪胃组织GHrmRNA水平高于大白猪 ,2 0日龄时差异显著 (P <0 0 5 ) ;90日龄至 180日龄期间二花脸猪胃组织GHrmRNA水平低于大白猪 ,90日龄时差异显著 (P <0 0 5 )。胃重相对于体重的变化模式和胃组织GHrmRNA水平的发育变化呈中等强度正直线相关 ,相关系数为r=0 5 4 (P <0 0 5 )。以上结果提示 :(1)猪胃组织中GHr基因表达有特定的发育模式 ,并且呈现显著的品种间差异 ;(2 )GH直接作用于胃组织并调节胃的生长 ,GH是否直接参与胃的功能性发育调节 。
Pituitary growth hormone (GH) is essential for postnatal growth in animal, it regulates numerous cellular functions by direct effect on it's receptor (GHr) in many different tissues. And it is believed that the abundance of GHr in different tissue determines the tissue sensitivity to GH. However, the regulation of GH on porcine stomach development is still unknown. So, in present experiment single tube semi quantitative RT PCR was applied to investigate the developmental patterns of gastric GHr mRNA expression in Large White and Erhualian Boars, and the Classic 18S internal standards (Ambion Inc.USA) was used. Pigs were sampled at birth,and 3, 20, 30, 45, 90, 120, 180 days of age respectively, meanwhile the bodyweight and gastric weight were recorded. The results indicated that the expression of gastric GHr mRNA was high at birth, followed by a significantly decrease ( P <0.05) at 3 days of age (D3) in both breeds. In Erhualian boars, GHr mRNA expression reached a peak at D45, then remarkablely declined till D90 ( P <0.05), and kept constant thereafter. In Large White boars, however, the expression of GHr mRNA reached a peak at D90 and remained pleateau afterwards. From birth to D30, the GHr mRNA expression was higher in Erhualian than that in Large White, the significant strain difference were founded at D20 ( P <0.05). But from D30 to D180, the higher expression was found in Large white,the significant strain difference was especially at D90 ( P <0.05). Significant positive correlation was found between the expression of GHr mRNA and the ratio of gastric weight to bodyweight from birth to D180 ( r =0.54, P <0.05). The results suggest that, (1) GHr mRNA in porcine stomach is expressed according a strain specific development pattern; (2) GH directly acts at the gastric tissue and regulates it's growth. The relationship between GH and the development of gastric function is to be elucidated.
基金
国家自然科学基金重点项目 (3 983 0 2 90 )~~
