摘要
目的 探讨乳癌 (MCF- 7)细胞株分泌物对凝血系统的影响 .方法 分别测定高低两种浓度的乳癌细胞分泌物对正常血浆和异常血浆的部分凝血活酶时间 (APTT) ,凝血酶原时间 (PT) ,凝血酶时间 (TT)值和 V ,V因子活性的影响 .结果 两种浓度的乳癌细胞分泌物均可使正常血浆APTT(s)显著缩短 (37.6± 0 .3) vs (31.0± 0 .5 ) ,(2 6 .0±0 .5 ) ,P<0 .0 5 ,而对 PT无影响 ,使异常血浆的 PT(s)延长(18.7± 0 .5 ) vs(2 3.6± 0 .5 ) ,(31.6± 0 .8) ,P<0 .0 5 ,而对APTT无影响 ;只有高浓度的分泌物能使 TT(s)延长 [正常血浆 (15 .8± 0 .6 ) vs(18.8± 0 .4 ) ,异常血浆 (18.8± 0 .5 ) vs(2 2 .4± 0 .5 ) ,P<0 .0 5 ].两种浓度的乳癌细胞分泌物均可使 V 因子活性增强 (12 5± 11) vs(170± 10 ) ,(2 5 0± 13) ,P<0 .0 5 ,只有高浓度的分泌物能使 V因子活性降低 (89± 10vs75± 9,P<0 .0 5 ) .
AIM To investigate the effects of secretions from breast cancer cell strain MCF 7 on blood coagulation system. METHODS Activated partial thromboplastin time (APTT), prothrombin time (PT), thrombin time (TT) and activities of factors VⅢ and V of normal and abnormal sera were tested respectively after influenced by higher and lower concentration secretions from breast cancer. REUSLTS Higher and lower concentration secretions shortened the normal serum's APTTs [(37.6±0.3) vs (31.0±0.5), (26.0±0.5), P <0.05], but exerted no effect on PT; the abnormal serum's PTs influenced by these two concentrations secretions were prolonged [(18.7±0.5) vs (23.6±0.5), (31.6±0.8), P <0.05], but the abnormal serum's APTTs didn't change. The higher concentration secretion might prolong TTs [normal serum (15.8±0.6) vs (18.8±0.4), abnormal serum (18.8±0.5) vs (22.4±0.5), P <0.05]. Secretions of higher and lower concentrations both enhanced the activity of VⅢ [(125±11) vs (170±10), (250±13), P <0.05], but the activity of V was decreased only by the higher concentration secretion [(89±10) vs (75±9), P <0.05]. CONCLUSION The secretion from breast cancer cell strain MCF 7 may influence the blood coagulation system.
出处
《第四军医大学学报》
北大核心
2002年第10期956-957,960,共3页
Journal of the Fourth Military Medical University
关键词
乳腺肿瘤
分泌物
血液凝固因子
breast neoplasms
bodily secretions
blood coagulation factors
