摘要
本论文利用Seliwanoff反应 ,在乙醇介质内测定了芥菜多糖中果糖的含量。果糖与间苯二酚之间生成的红色络合物在 4 73nm处吸收峰最高 ,在 0～ 83μg·mL- 1 范围内符合比耳定律 ,A =0 0 2 8+0 0 2 0 2 2c ,r =0 9998。测得芥菜多糖中含有 11 4 1%的果糖。
A method using seliwanoff reaction is proposed for the determination of fructose in the amylose of mustard leaves. Fructose reacts with resorcinol forming a red compound with a maximum absorption at 473 nm. The calibration curve is linear over the range of 0-83 mug . mL(-1) with a correlation coefficient of 0.999 8. The content of fructose in the amylose of mustard leaves is found to be 11.41%. Quntitative analysis used for the fructose of syrupus include such as volumetric analysis, then layer chromatography, polarimetry, high pressure liquid chromatography and spectrophotometric analysis. According to different color-developing agents, spectrophotometric analysis may be classified as carbazole method, ammonium molybdate way and resorcinol way etc. reported by foreign papers is hard to operate and colored complex is unstable.
出处
《光谱学与光谱分析》
SCIE
EI
CAS
CSCD
北大核心
2002年第3期446-448,共3页
Spectroscopy and Spectral Analysis
关键词
芥菜
多糖
果糖
间苯二酚
分光光度法
测定
mustard leaf of amylose
fructose
content
spectrophotometer
