摘要
从蒺藜(Tribulus terristris L)茎和叶中提出醇溶皂甙后,再提取水溶性粗多糖,其单糖组成及其摩尔比为Ara.,Rha.,Xyl.,GalA.,Gal.,Glc.,Man.,6.0:2.1:1:3.6:3.4:7.7:2.9.粗多糖经分级与纯化得一均一级分H,其单糖组成及其摩尔比为Ara.,Rha,Xyl,GalA,Gal,Glc,1.6:2.4:0.1:3.5:1.3:1。H为酸性杂多糖,分子量约为10万。经果胶酶酶解、β-D-半乳糖苷酶酶解,高碘酸氧化、Smith降解、部分酸水解、甲基化、与GC及GC-MS分析等,表明H为α-D-GalA(1→4)与L-Rha(1→2)可能交替连接构成的主链,一些Rha.上带有分枝。
Crude polysaccharides extracted from the stem and leaf of Tribulus terristris L after the removal of crude saponins are a mixture of heteropolysaccharides composed of Am ,Rha, Xyl ,GalA, Gal,Glc and Man in molar ratios of 6. 0:2. 1:1:3.6 :3.4:7.7:2.9.A homogeneous polysaccharide H obtained by gradation and purification contains Am, Rha, Xyl, GalA. Gal and Glc. in molar ratios of 1.6: 2.4: 0. 1: 3.5: 1.3: 1. Its molecular weight was found to be 1×10~5.By means of pectinase and β-D-galactosidase enzymolysis, periodate oxidation,Smith degradation, partial hydrolysis withacid, methylation, GC and GC- MS, the H Contains α - D- GalA ( 1 - 4 ) and L- Rha ( 1 - 2 ) probably linked alternately as main chain with some L- Rha ( 1 - 2 ) side chains.
出处
《药学学报》
CAS
CSCD
北大核心
1991年第8期578-583,共6页
Acta Pharmaceutica Sinica
基金
自然科学基金
关键词
蒺藜
杂多糖
肝炎
性功能
Tribulus terrestris L
Tribulus terrestris polysaccharide
Heteropoly charide
