摘要
本文以抗病自交系黄野四 - 3、感病自交系 8112和 Mo17、耐病自交系 4 78为材料 ,采用直接组织斑免疫测定法 (IDDTB)和酶联免疫吸附测定法 (ELISA)结合生物学症状研究了玉米矮花叶病毒对玉米的侵染及其运转规律 ,试验表明玉米抗玉米矮花叶病毒主要是抗系统传导 ,其次抗病毒增殖和细胞间扩展 ,但不抗侵入。
The infection and movement of Maize dwarf mosaic virus strain B (MDMV B) in corns were studied using resistant inbred (Huangyesi 3), susceptible inbreds (8112 and Mo17) and tolerant inbred (478). Immunological detection of direct tissue blotting (IDDTB) and enzyme linked immunosorbent assay (ELISA) were applied to detect MDMV in inoculated corns and the disease symptoms were investigated. The results show that the resistance of maize to MDMV B is due to prevention of the systemic spread and replication of virus, but not the virus infection.
出处
《植物病理学报》
CAS
CSCD
北大核心
2002年第2期153-158,共6页
Acta Phytopathologica Sinica
基金
河北省自然科学基金资助项目 (395 2 84 )
