摘要
Hybridoma cells were cultured by continuous perfusion in Fibra-Cel of 5L packed-bed bioreactor for 22 days in low serum or serum-free media.The corresponded amino acids were fed and serum concentration was decreased by analyzing glucose concentration, oxygen uptake rate, secretary antibody amount and amino acids concentration in culture supernatant. Comparing with continuous perfusion culture that amino acids were not fed, antibody amunt of production was increased about 2～3 times. The inoculated cell density was 2.5×10 5 cells/mL,while the final cell density was 8.79×10 8cells/mL. Antibody production was reached 295mg/L/d at average level, and the highest level was reached 532mg/L/d.These results provided a primary mode of enlarge culture for monoclonal antibody industrilization.
Hybridoma cells were cultured by continuous perfusion in Fibra-Cel of 5L packed-bed bioreactor for 22 days in low serum or serum-free media.The corresponded amino acids were fed and serum concentration was decreased by analyzing glucose concentration, oxygen uptake rate, secretary antibody amount and amino acids concentration in culture supernatant. Comparing with continuous perfusion culture that amino acids were not fed, antibody amunt of production was increased about 2～3 times. The inoculated cell density was 2.5×10 5 cells/mL,while the final cell density was 8.79×10 8cells/mL. Antibody production was reached 295mg/L/d at average level, and the highest level was reached 532mg/L/d.These results provided a primary mode of enlarge culture for monoclonal antibody industrilization.
出处
《生物工程学报》
CAS
CSCD
北大核心
2002年第3期360-364,共5页
Chinese Journal of Biotechnology
基金
国家九五科技攻关重点资助项目 (No .96 90 1 0 1 0 8)~~
关键词
连续灌流培养
杂交瘤细胞
单克隆抗体
perfusion culture, hybridoma, monoclonal antibody, high density
