摘要
目的 探讨人血清白蛋白 (humanserumalbumin ,HSA)对人牙龈上皮细胞 (humangingivalepithelialcells ,HGE)粘附作用的影响。方法 使用角化细胞无血清培养基分离酶原代培养HGE ,并用广谱细胞角蛋白单抗做细胞鉴定 ,细胞接种 2 4h内用MTT法连续观测HSA聚苯乙烯表面预孵育组及培养基含HSA组HGE的粘附 ,在含HSA培养基中观测HGE的生长曲线。结果 HGE免疫组化染色阳性 ;HSA预孵育组 8h以内粘附的细胞数量显著少于培养基含HSA组及对照组 ,10～ 2 4h两实验组与对照组间差异无显著性 ;生长曲线中各时间点细胞的数量 ,实验组与对照组相比差异无显著性。结论 HSA预孵育于聚苯乙烯表面 。
Objective To investigate the effect of human serum albumin(HSA)on cell attachment of human gingival epithelial cells(HGE). Methods HGE were primary cultured with keratinocyte serum free medium(KSFM) and dispase.The cultured cells were immunohistochemically stained by monoclonal anti pan cytokeratin.MTT test was employed to investigate the influence of HSA on the cell attachment on polystyrene surface. The cell growth curve of HGE which were cultured in KSFM with 50 g/L HSA was observed. Results The results showed significant decrease in cell numbers within 8 hours after HGE were inoculated,in which the polystyrene surface was preincubated with 50 g/L HSA. But it did not prove to be the case from 10 hours to 24 hours after HGE were inoculated.There were no significant difference within 24 hours in cell numbers between cultured in KSFM with 50 g/L HSA and control.The cell numbers in cell growth curve of HGE in KSFM with and without 50 g/L HSA did not show significant difference. Conclusions HSA preincubation on polystyrene were produce inhibitory effect of HGE attachment in early stage.
出处
《中华口腔医学杂志》
CAS
CSCD
北大核心
2002年第3期197-199,W005,共4页
Chinese Journal of Stomatology
基金
国家自然科学基金资助项目 (3 9870 2 19
3 970 0 164 )
