摘要
目的 利用油酸 (OA )复制家兔急性肺损伤模型 ,观察硫酸镁的防治作用并探讨其机制。 方法 2 4只兔随机分为 3组 (各 8只 ) : .对照组 ; .油酸损伤组 ,按 0 .12 ml/ kg静推 OA,损伤前 6 0 min静推生理盐水 ; .硫酸镁防治组 ,损伤前 6 0 min按 2 m g· kg- 1 · min- 1 推注 Mg SO4直至实验结束。于损伤前 6 0 m in,损伤后 6 0 ,12 0 ,180 min抽颈总动脉血查氧分压、L PO、SOD活力 ,实验末取肺标本观察病理改变 ,计算肺系数。 结果 (1)油酸损伤组和硫酸镁防治组在 OA损伤后 Pa O2 呈进行性下降 ,与损伤前比较差别有显著意义 (P<0 .0 1) ;但防治组Pa O2 下降的幅度比损伤组小 ,在伤后 6 0 ,12 0和 180 min Pa O2 测定损伤组比对照组下降了 30 .7% ,35 .5 %和40 .6 % ,而防治组为 13.6 % ,16 .6 %和 19.6 % ,两组差别有显著意义 (P<0 .0 1)。 (2 )损伤组 L PO含量与对照组比各时点分别增加了 6 4.7% ,80 .5 %和 95 .5 % ,差别显著 (P<0 .0 1) ;防治组增加不如损伤组明显。 (3)损伤组 SOD与对照组相比 ,各时点分别下降 32 .6 % ,40 .2 %和 49.2 % (P<0 .0 1) ;防治组下降不如损伤组明显。 (4 )损伤组和对照组肺系数分别比对照组高出 12 3.8%和 40 .4% (P<0 .0 1) ,但防治组不如损伤组明显 ;
Objective This study utilized the model of acute lung injury induced by oleic acid(OA) in rabbits for research the protective effect of magnesium sulfate and its possible mechanism. Methods 24 rabbits were randomly divided into 3 groups, 8 rabbits in each, including group Ⅰ, normal control, Ⅱ, injurious(OA, 0 12 ml/kg) and Ⅲ, protective MgSO 4+OA. In group Ⅲ, rabbits were injected MgSO 4(2 mg·kg -1 ·h -1 ) from 60 minutes before injury to the end of experiment. Blood samples were taken from cervical artery 60 min before injury and 60, 120, 180 min after injury for determination of PaO 2, LPO, SOD. At the end of experiment, the lungs were observed for pathologic changes by naked eyes and under microscope, and lung fraction was calculated. Results (1)In group Ⅱ and Ⅲ, PaO 2 decreased gradually after injury compared to those of before(P<0 01). At 60, 120 and 180 min after injury, PaO 2 in group Ⅱ and Ⅲ decreased 30 7%, 35 5% and 40 6%; and 13 6%, 16 6% and 19 6% respectively as compared to those of group Ⅰ. The difference between group Ⅱ and Ⅲ was significant(P<0 01). (2)In contrast to group Ⅰ, the LPO contents in blood increased 64 7%, 80 5% and 95 5% in group Ⅱ at 60, 120 and 180 min after injury(P<0 01). In group Ⅲ, they were higher than those of group Ⅰ(P<0 01) but lower than group Ⅱ(P<0 01). (3)The activity of SOD in blood decreased 32 6%, 40 2% and 49 2% in group Ⅱ at 60 120 and 180 min after injury as compared to that of group Ⅰ(P<0 01). (4)Lung fraction increased 123 8% in group Ⅱ and 40 4% in group Ⅲ as compared to those of group Ⅰ(P<0 01). In group Ⅲ, it was significantly lower than that of group Ⅱ(P<0 01). Comparing the pathologic changes of lungs, the degree of injury in group Ⅲ was lesser than that in group Ⅱ. Conclusion In OA induced acute lung injury of rabbits, magnesium sulfate could protect the lung injury through the mechanism of inhibiting oxide free redicals production.
出处
《福建医科大学学报》
2002年第1期56-58,共3页
Journal of Fujian Medical University
关键词
硫酸镁
肺损伤
油酸类
氧
自由基
兔
magnesium sulfate
lung injury
oleic acid
oxide
free redicals
rabbit
