摘要
目的 探讨NOEY2基因与胰腺癌的相关性。方法 病理确诊的胰腺肿瘤26例（23例胰腺导管腺癌、3例胰岛细胞瘤）,12例正常胰腺,8株人胰腺癌细胞株（Pu-Pan-1、Miapaca-Ⅱ、Panc-1、CFpac-1、Aapc-1、BXPC3、HS766T、SW1990）。用NOEY2特异性抗体进行免疫组化检测NOEY2基因编码蛋白在胰腺癌组织的表达;用该基因的cDNA探针进行RNA印迹杂交分析上述胰腺癌细胞株有无NOEY2 mRNA表达;用免疫细胞化学的方法检测NOEY2基因编码蛋白在胰腺癌细胞株的表达。结果 （1）正常人胰腺导管、腺泡、胰岛细胞胞浆中均可见到NOEY2蛋白的表达;（2）23例胰腺导管腺癌组织中有11例胰腺癌组织无NOEY2蛋白表达,占47.8％,又可分为二种类型,一种为肿瘤组织表达阴性、正常组织表达阳性（2/11）,另一种为肿瘤组织、正常组织表达均阴性（9/11）;12/23例（52.2％）肿瘤组织癌旁组织表达均阳性。（3）本实验未发现NOEY2蛋白与胰腺癌分化程度之间有相关性;（4）3例胰岛细胞瘤均无NOEY2蛋白表达;（5）RNA印迹杂交分析及免疫细胞化学结果显示8个胰腺癌细胞株均无NOEY2基因在mRNA和蛋白水平的表达。结论 NOEY2基因蛋白在正常人胰腺广泛表达,但在胰腺癌组织中蛋白表达有较高比例的缺失,在胰腺癌细胞株中不仅有蛋白表达缺失,同时NOEY2基因mRNA表达?
Objective The aim of this study was to disclose the association of NOEY2 with pancreatic carcinoma. Methods Twenty-six human pancreatic carcinoma tissue specimens (23 cases of pancreatic adenocarcinoma, 3 cases of insulinoma), 12 normal pancreatic tissues and 8 human pancreatic carcinoma cell lines (Pu-Pan-1, Miapaca-II. Panc-1 , Cfpac-1, Aapc-1 , BXPC3, HS766T, SW1990) were collected. The final diagnoses of all the cases were confirmed by a senior pathologist. The expression of NOEY2 protein in pancreatic carcinoma and normal pancreatic tissue was detected by immunohistochemistry method, and the expression of NOEY2 protein and mRNA in the cell lines were detected by immunocytochemistry and Northern blot hybridization method. Results (1) NOEY2 protein was expressed in the cytoplasm of normal pancreatic ducts cells, pancreatic acini cells and islet cells. (2) The NOEY2 protein was found in 12/23 pancreatic adenocarcinoma tissues (both carcinoma tissue and adjacent tissue). No NOEY2 protein was detected in 11/23 pancreatic adenocarcinoma tissue, which consisted of two types; NOEY2 protein was detected from 2/11 carcinoma adjacent tissue but not from pancreatic carcinoma tissues, in other 9/11, no NOEY2 protein was detected from pancreatic carcinoma tissues or carcinoma adjacent tissue. (3) The expression of NOEY2 protein was not correlated with the grade of differentiation of pancreatic adenocarcinoma. (4) NOEY2 protein was not found in 3 insulinoma tissue. (5) Neither NOEY2 protein nor mRNA was detected in any of the 8 pancreatic carcinoma cell lines. Conclusions NOEY2 is a possible tumor suppressor gene which may play a role in the pathogenesis of pancreatic carcinoma.
出处
《胰腺病学》
2002年第1期28-30,共3页
Chinese JOurnal of Pancreatology
