摘要
目的 研究复方红芪提取液的作用机制。方法 分别使用 1 60 0、3 2 0 0、6 40 0、1 2 80 0倍稀释的复方红芪提取液 ,培养新鲜切取的纯系SD大鼠坐骨神经 ,于培养后 2 4、48和 72h,使用[γ 32 P]ATP掺入法 ,观察雪旺细胞中蛋白激酶A活性变化。使用神经生长因子作阳性对照 ,正常培养液作阴性对照。结果 正常培养条件下 ,离体神经中雪旺细胞的活化蛋白激酶A比率在 2 4、48、72h时分别 7.42、0 .1 7、0 .0 7;神经生长因子组分别为 73 .70、62 9.71、44 .64 ;红芪 1 60 0稀释组分别为 842 .1 1、1 33 .40、1 3 .2 6 ;3 2 0 0稀释组分别为 1 30 .1 6、1 0 7.37、1 6 .2 1 ;6 40 0稀释组分别为88.76、1 9.2 3、2 .67;1 2 80 0稀释组分别为 1 50 .51、63 .0 8、2 1 .50。分析以上数据显示 ,神经生长因子组与红芪组均可见蛋白激酶A活性显著增加 ,后者呈明显的时间负相关及浓度正相关。秩和检验不同浓度的红芪提取液及不同的作用时间 ,对蛋白激酶A活性的影响差异均有非常显著性 (P<0 .0 0 5)。结论 复方红芪提取液促进神经修复的功能是通过G蛋白、环磷酸腺苷、蛋白激酶A途径促进雪旺细胞增生 。
Objective To study the mechanism of extracts of Hedysarum Ploybotrys Hand. Ma22 on schwann cell.Methods Sciatic nerves were obtained by operation from SD rats and cultured in PRMI 1640 medium with 15% embryo bovine serum and with Hedysarum Ploybotrys Hand. Ma22 at a dilution of 1∶1?600,3?200,6?400,12?800 or with nerve growth factor (NGF).NGF served as positive control and normal medium as negative one.After 24,48 and 72?h cultures,the sciatic nerves were captured separately and used to determine the activity of PKA by γ 32 P ATP incorporation test using protein kinase A assay system.Results The ratio of active PKA was 7.42,0.17,0.07 at 24,48 and 72th h respectively in the normal culture,73.70,629.71,44.64 in the NGF group, 842.11,133.40,13.26 in the Ma22 group with dilution of 1?600;130.16,107.37,16.21 Ma22 group with dilution of 3?200; 88.76, 19.23,2.67 in the Ma22 group with dilution 6?400;150.51,63.08,21.50 in the Ma22 group with dilution 12?800.From those data,in the NGF group and Ma22 groups,the activity of PKA was notably increased.In the group of Hedysarum Ploybotrys Hand. Ma22,the correlations to time were negative and to drug concentrations positive.The results of rank sum test showed the difference was significant (P<0.005).Conclusion Extracts of the Hedysarum Ploybotrys Hand. Ma22 can promote the growth of Schwann cells by receptor cAMP PKA signal pathway.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2002年第3期281-282,共2页
Chinese Journal of Experimental Surgery
基金
国家自然科学基金资助项目( 39870 851)
