摘要
目的观察家兔羊水栓塞 (AFE)后肺组织中肥大细胞类胰蛋白酶 (MCT)含量的变化。方法分别于健康怀孕家兔耳缘静脉注入羊水、制造羊水栓塞的动物模型 ;取肺组织进行HE染色和免疫组织化学MCT染色 ,镜下观察AFE兔肺组织中MCT的变化。结果对照组兔肺组织MCT呈弱阳性反应 (平均阳性细胞计数 11 2 5个 ) ,注入羊水组和注入胎盘提取液与羊水混合液组肺组织中MCT呈强阳性反应 (平均阳性细胞计数分别为 3 2 98和 45 5 3个 )。
Objective To observe the number of pulmonary mast cell tryptase(MCT) after amniotic fluid embolism(AFE) in experimental rabbits.Method The experimental amniotic fluid embolism rabbits model was established by injecting the amniotic fluid(AF) or placental extracts mixed with amniotic fluid(PAF)into the marginal vein of the pregnant rabbits.Immunohistochemistry and microscopy were employed to observe the number of pulmonary MCT in AFE rabbits.Results A marked increase in MCT positive cells was observed in lungs in both AF and PAF groups(average cell number were 32 98 and 45 53respectively)as compared with the control group(average cell number was 11 25).Conclusion The results indicated that the number of MCT cells were marked increased in case of the AFE.
出处
《中国法医学杂志》
CSCD
2002年第1期4-6,共3页
Chinese Journal of Forensic Medicine
基金
山西省教委青年学科带头人基金 (No :980 0 6)
关键词
法医病理学
羊水栓塞
免疫组织化学
肥大细胞类胰蛋白酶
Forensic pathology
Amniotic fluid embolism (AFE)
Immunohistochemistry
Mast cell tryptase(MCT)
