摘要
采用植物血凝素（PHA）致敏S、D大鼠，2周后处死，灌洗腹腔，用阿拉伯胶分离液分离肥大细胞（MC），将PHA与MC在37C水治中孵育，攻击MC使之受损伤，于伤后5、10、15、20、30和60分钟，分别吸取孵有液作聚丙烯酰胺凝胶圆盘电泳，分离MC过氧化物酶；用抗坏血酸-联苯胺染色显带，并计算酶的相对活性。另取20分钟时孵育液，常规固定，包埋，切片及染色后用透射电镜观察MC脱颗粒叶的形态。用常规石蜡切片，甲苯胺蓝染色，对SD大鼠的35种组织中MC的分布进行光镜观察。结果发现；（1）大鼠腹腔MC显示-条过氧化物酶电泳区带；（2）MC经PHA攻击受损伤10分钟内，过氧化物酶相对活性升高不明显；15、ZO、3O分钟时实验组酶活性升高，与对照组的差异明显；到60分钟时两组酶活性无显著差异；（3）MC与PHA孵育20分钟时，脱颗粒率为76％，MC浆内可见有膜性和无膜性两种类型的空泡．对照组大鼠MC脱颗粒仅为16％；（4）大鼠皮肤、肺、呼吸道、消化道、骨髓及胸腺MC含量较多；泌尿生殖道次之；心、肝、脾、肾等组织较少；脑、脊髓、脑垂体、肾上腺及睾丸等组织中未见MC，从而为法医损伤时间的推测提供方法与依据。
S. D rats were sensitized with PHA and were killed after two weeks. Peritoneal MC were collect-ed and separated using Arabic gum separating solution. Those MC were attacked by adding PHA andfol1owed by incubating at 37 °C in water bath. After incubating for 5, 1O, l5, 2O, 30min and Ih,eachincubating medium was aspirated. The MC peroxidase was separated by disc electrophoresis and wasstained by ascrobic acid-benzidine stain. The relative peroxidase activity was calculated. Mean while,1O drops of 20 minute incubating medium were aspirated, fixed,embeded,sectioned,stainedand exam-ined under the electronic microscope routinely- 35 different kinds of 10 S. D. rat, s tissues were cut,embeded,sectioned,and stained with the toluidine blue and examined under light microscope for ob-serving the tissue distribution of MC. The results were as follows: (l )The peroxidase released fromthe PHA attacked MC was successfully demonstrated; (2)As comparing with the c0ntr0l group, theincreasing of the relative peroxidase activity in experirnental groups was not obvious at the intervals of5min and 10min incubating, but was obvious as the intervals of 15~3Omin incubating. No significantincreasing of the relative peroxidase activity was found at 60min incubating1 (3)When incubating for2Omin,the degranulation ratio of the experimental groups was 76%,while that of the control groupswas l6% only. There were two kinds of vacuoles in the degranulating MC plasrna, vacuoles withmembrane and without rnembrane. (4 ) The MC in skin, lung, respiratory tract, digestive tract, boneand thymus were prevalent. The order of MC number decreasing was as follows:urinary tract,repro-ductive tract, heart, liver, spleen, kidney. No MC was found in brain, spinal cord, hypophysis andtestls.
出处
《中国法医学杂志》
CSCD
1995年第4期196-200,共5页
Chinese Journal of Forensic Medicine
