摘要
目的 探讨蝙蝠葛碱 (DAU )对急性心肌梗死 (AMI)患者血小板不可逆性聚集的作用。方法 用流式细胞仪测定AMI患者 (n =12 )静息状态及凝血酶 (5 0、10 0、5 0 0、10 0 0U·L-1)激活时 ,血小板膜表面糖蛋白Ⅳ (GPIV)及凝血酶敏感蛋白 (TSP )分布状况 ,并与健康人 (n =14)比较。结果 AMI患者静息及激活状态血小板膜表面GPⅣ ,TSP分布高于健康人 (P <0 0 5 ,0 0 1) ;DAU(5 0 μmol·L-1)可抑制 5 0U·L-1,10 0U·L-1及 5 0 0U·L-1凝血酶诱导的血小板膜GPⅣ再分布及血小板内TSP释放 (P <0 0 5 ,0 0 1) ,不能抑制10 0 0U·L-1凝血酶诱导的血小板膜GPⅣ再分布及血小板内TSP释放。结论 AMI患者血小板活性及对凝血酶的反应性增高 ,DAU可减少AMI患者血小板不可逆性聚集的发生。
AIM To investigate the possibility of dauricine (DAU) to inhibit the irreversible platelet aggregation of patients with acute myocardial infarction (AMI). METHODS GlycoproteinⅣ (GPⅣ) and thrombospondin(TSP) levels on the membrane surface of the stationary platelet or platelet activated by thrombin(50?100? 500? 1 000 U·L -1 ) in 12 patients with AMI were measured with a washed platelet flowcytometric assay and compared with those of the healthy ( n =14). RESULTS The GPⅣ, TSP level of stationary platelet and activated platelet in patients with AMI were higher than those in the healthy significantly( P <0 05, 0 01), DAU (50 μmol·L -1 ) inhibited the GPⅣ membrane redistribution and the release of TSP from intracellular α granules induced by thrombin (50 U·L -1 , 100 U·L -1 , 500 U·L -1 ) apparently ( P <0 05, 0 01), inhibitory effect of DAU was not found in platelets activated with high concentraction of thrombin ( 1 000 U·L -1 ). CONCLUSION The activity and reactivity to thrombin of platelets increased in patients with AMI and DAU may have a blocking effect on the consolidation of platelet aggregation in AMI.
出处
《中国药理学通报》
CAS
CSCD
北大核心
2002年第1期97-99,共3页
Chinese Pharmacological Bulletin
关键词
蝙蝠葛碱
急性心肌梗死
血小板聚集
凝血酶
dauricine
acute myocardial infarction
platelet aggregability
thrombin
