摘要
背景及目的:近年的研究发现一氧化氮在肿瘤的发生、发展中起着重要的作用,在头颈肿瘤中检测出高水平的诱导型一氧化氮合酶(induciblenitricoxidesynthase,iNOS)表达,并与肿瘤的进展正相关,未见有关口腔癌中iNOSmRNA与其生物学行为相关的报道。本文拟研究舌鳞癌组织中iNOSmRNA表达与其侵袭性生长、颈淋巴结转移、预后的相关性。方法:原位杂交检测常规石蜡包埋的68例舌鳞癌活检组织中iNOSmRNA的表达;对所研究的舌鳞癌病例进行随访,获取有关预后资料;用原位杂交技术来分析iNOSmRNA与舌鳞癌侵袭性生长、颈淋巴结转移、预后的相关性。结果:①舌鳞癌浸润肌层组与无肌层浸润组iNOSmRNA阳性率分别为86.1%、48.0%,两组间差异有显著性(P<0.05),相关系数为0.3。②iNOSmRNA在舌鳞癌有颈淋巴结转移组阳性率为85.7%,无颈淋巴结转移组阳性率为62.5%,两组间差异有显著性(P<0.05),相关系数为0.3。③舌鳞癌iNOSmRNA表达阳性组3年生存率显著低于iNOSmRNA表达阴性组(P<0.05)。结论:①iNOSmRNA阳性表达与舌鳞癌的侵袭性生长、颈淋巴结转移正相关。②舌鳞癌组织中iNOSmRNA表达阳性的病例较iNOSmRNA表达阴性的病例预后差。
Background &Objective:Recently,most of studies have indic ated that nitric oxide(NO)plays an important role in carcinogenesis an d tumor progression.Inducible nitr ic oxide synthase(iNOS)has been found at significantly higher expression levels in head and neck squamous cell carcinoma (HNSCCA)specimens as compared with that in normal tissue.Furthermore,these data pro vided strong evidance for the role of iNOS generating NO in HNSCCA progression.However,there was no report about whether expression of iNOS mRNA in oral carcinoma is associated with its bio logical behavior.This study was designed to investigate the correlation between expression of inducible nitric oxide synthase mRNA(iNOS mRNA)in squamous cell carcinoma of tongue tissue and its invasive growth,neck lymph node metastasis,and prognosis.Methods:Sixty-eight cases with squamous cell carcinoma of tongue tissues were investigated.All cases with tongue carcinoma were followed up to obtain the information of prognosis.In situ hybridization assay was used to detect the expression of iNOS mRNA in tongue carcinoma tissues.The correlation between expression of i NOS mRNA in squamous cellcarcinoma of tongue tissue and its in vasive growth,neck lymph node metastasis,and prognosis was analyzed.Results:①The positive expression rate of iNOS mRNA was 86.1%and 48.0%in the group of in vasive growth and no invasive growth respectively.The difference betwe en the two groups was significant with coefficient of 0.3.②The positive expression rate of iNOS mRNA was 85.7%and 62.5%in the group of positive lymph node metastasis and negative lymph node metastasis respectively.The difference betwe en the two groups was significant,which the correlation coefficient was0.3.③Three-year survival rate was significantly low er in the group of positive expressio n of iNOS mRNA than that in the group of negative expression of iNOS mRNA in the tongue carcinoma.Conclusions :①A positive correlation was found between the positive expression of iNOS mRNA and invasive growth as well as ne
出处
《癌症》
SCIE
CAS
CSCD
北大核心
2002年第3期314-318,共5页
Chinese Journal of Cancer
基金
广东省卫生厅基金资助项目(A2000207)