摘要
为探究复合微生物菌剂对苹果再植病害的生防效果,以苹果砧木实生海棠幼苗为试材,通过盆栽试验测试了木美土里复合微生物菌剂对苹果再植病害的防治效果及对再植海棠的促生作用,然后对该菌剂的可分离微生物进行了分离纯化,并以尖孢镰刀菌Fusarium oxysporum菌株HS2为靶标菌,测试分离获得的10株菌株的拮抗效果,并对拮抗效果显著的1株真菌菌株和2株细菌菌株进行种类鉴定。结果表明,木美土里复合微生物菌剂对苹果再植病害的防治效果达87.78%;经该菌剂处理后,再植海棠苗的株高、茎粗、鲜重、干重及叶面积分别是对照处理的1.51、2.00、5.74、5.21和2.83倍,表明该菌剂有显著的促生作用。木美土里复合微生物菌剂对菌株HS2的抑制率达到68.59%,并从中分离到7株真菌菌株,3株细菌菌株。其中,拮抗效果高于80.00%的真菌菌株有1株,细菌菌株有2株。经鉴定,真菌菌株MMTL-1为哈茨木霉Trichoderma harzianum;细菌菌株BM-01和BM-03均为解淀粉芽胞杆菌Bacillus amyloliquefaciens。
To study the bio-control effect of compound microbial against apple replant disease, pot experiments were applied to evaluate the control effect of Kimidori microbial manure (KMM) against the apple replant diseases (ARD) and whether promoting plant growth of Malus seedlings. Ten microorganism strains isolated from KMM were tested in vitro for their antagonistic activity against Fusarium oxysporum, one of them was the inducing factor of ARD. The species with better inhibition rate were identified through morphology and molecular biology methods. The results showed that the control effect of KMM against ARD could reach 87.78%. Seedling growth was significantly promoted by KMM treatment. The plant height, stem diameter, fresh weight, dry weight and leaf area of KMM treatment increased 1.51, 2.00, 5.74, 5.21 and 2.83 times compare with that of the replant control. The inhibition rate of KMM against F. oxysporum HS2 was 68.59%. Seven fungal isolates and three bacterial strains were isolated from KMM. Among these isolates, one fungal isolate MMTL-1 with the inhibition rate over 80.00% was identified as Trichoderma harzianum. Both the two bacterial isolates BM-01 and BM-03 with the inhibition rate over 80.00% were identified as Bacillus amyloliquefaciens.
作者
赵璐
刘胜
胡同乐
王亚南
王树桐
曹克强
Zhao Lu;Liu Sheng;Hu Tongle;Wang Yanan;Wang Shutong;Cao Keqiang(College of Plant Protection,Hebei Agricultural University,Baoding 071000,Hebei Province,China)
出处
《植物保护学报》
CAS
CSCD
北大核心
2019年第1期208-215,共8页
Journal of Plant Protection
基金
国家重点研发计划(2016YFD0201100)
河北省自然科学基金(c2016204140)
国家现代农业(苹果)产业技术体系(CARS-27)
关键词
复合微生物菌剂
苹果再植病害
生物防治
哈茨木霉
解淀粉芽胞杆菌
microbial manure
apple replant diseases
biological control
Trichoderma harzianum
Bacillus amyloliquefaciens
