摘要
目的研究益气活血方对内毒素(lipopolysaccharide,LPS)诱导内皮细胞炎症反应的干预作用。方法体外培养内皮细胞EA. hy926,设为空白对照组、LPS组、益气活血方低剂量组、益气活血方高剂量组、辛伐他汀组、SB203580(p38 MAPK抑制剂)组、SB203580+益气活血方组,药物预处理3 h后加LPS刺激12h,收集上清及细胞,ELISA检测上清炎性因子TNF-α、IL-6、IL-8水平,Western blot检测黏附因子VCAM-1、ICAM-1蛋白表达水平及p38 MAPK蛋白磷酸化水平。结果与空白对照组比较,LPS组TNF-α、IL-6、IL-8分泌水平以及VCAM-1、ICAM-1蛋白表达水平升高(P <0. 01),p38 MAPK蛋白磷酸化水平亦明显升高(P <0. 01);与LPS组比较,益气活血方低、高剂量组、辛伐他汀组TNF-α、IL-6、IL-8、VCAM-1、ICAM-1表达水平以及p38 MAPK磷酸化水平明显降低(P <0. 05,P <0. 01);益气活血方组、SB203580组以及益气活血方+SB203580组TNF-α、IL-6、IL-8、VCAM-1、ICAM-1表达水平及p38 MAPK磷酸化水平较LPS组均有明显下降(P <0. 05,P <0. 01),益气活血方+SB203580组与益气活血方组比较,差异无统计学意义(P> 0. 05)。结论益气活血方通过抑制p38 MAPK活性发挥抗LPS诱导内皮细胞炎症反应之作用。
Objective To study the effects of Yiqi Huoxue Formula( YQHX) on inflammatory responses induced by lipopolysaccharide( LPS) in endothelial cells. Methods Endothelial cells were culturedin vitro and divided into seven groups,including control group,LPS group,low-dose group of YQHX,high-dose group of YQHX,simvastatin group,SB203580( p38 MAPK inhibitor) group,and SB203580 plus YQHX group. After pretreated with above chemicals for 3 h,LPS was added into endothelial cells and incubated for another 12 h,and supernatant and cells were collected,respectively. The levels of TNF-α,IL-6 and IL-8 in the supernatant were detected using ELISA,and the expression of VCAM-1 and ICAM-1 as well as the phosphorylation level of p38 MAPK were determined using Western blot. Results The levels of TNF-α,IL-6,IL-8,VCAM-1 and ICAM-1 in LPS group were higher than those in control group(P <0. 01),and the phosphorylation level of p38 MAPK was also increased significantly(P<0. 01). Compared with the LPS group,the levels of TNF-α,IL-6,IL-8,VCAM-1,ICAM-1 and p38 MAPK phosphorylation were decreased in low-dose and high-dose group of YQHX group and simvastatin group(P <0. 05,P <0. 01). The levels of TNF-α,IL-6,IL-8,VCAM-1 and ICAM-1 and the expression of p38 MAPK in SB203580 group and SB203580 plus YQHX group were also dramatically reduced(P <0. 05,P < 0. 01). There was no significant difference among groups of YQHX,SB203580 and SB203580 plus YQHX(P >0. 05). Conclusion YQHX suppresses the inflammatory responses in endothelial cells induced by LPS by inhibiting the activity of p38 MAPK.
作者
吴鸿
王新洲
高水波
雷震
高海霞
韩勇军
王振涛
韩丽华
WU Hong;WANG Xin-zhou;GAO Shui-bo;LEI Zhen;GAO Hai-xia;HAN Yong-jun;WANG Zhen-tao;HAN Li-hua(Laboratory of Cell Imaging,Henan University of Chinese Medicine, Zhengzhou (450002);Institute of Cardiovascular Disease,Henan University of Chinese Medicine, Zhengzhou (450002))
出处
《中国中西医结合杂志》
CAS
CSCD
北大核心
2019年第2期200-205,共6页
Chinese Journal of Integrated Traditional and Western Medicine
基金
国家自然科学基金资助项目(No.81473453
81673800)
河南省高校科技创新人才支持计划(No.14HASTIT028)
河南省科技攻关项目(No.142102310040)
关键词
益气活血方
内毒素
内皮细胞
炎性因子
黏附因子
Yiqi Huoxue Formula
lipopolysaccharide
endothelial cells
inflammatory factors
adhesion factor
