摘要
目的建立三维同步荧光光谱法测定黄芪中芒柄花素含量的方法。方法通过扫描三维同步荧光光谱,确定同步荧光光谱的最佳波长间隔,用同步荧光测定不同产地的黄芪中芒柄花素含量。结果同步荧光光谱的最佳波长间隔为130 nm,芒柄花素在0.005~1.28μg·m L^(-1)范围内同步荧光强度与发射波长具有良好的线性关系,标准曲线的回归方程为:IF=252.554 62c+4.953 09,r=0.999 6。结论该方法重复性良好,准确度高,检测限低,结果可靠,可用于黄芪中微量芒柄花素的测定。
Objective To establish the determination method of formononetin in Astragali radix with three-dimensional synchronous fluorescence spectrometry.Methods Three-dimensional synchronous fluorescence spectra were used to select the best wavelength interval,and formononetin contents in Astragali radix of different resources were determined by synchronous fluorescence spectrometry.Results The wavelength spacing of 130 nm was identified by threedimensional synchronous fluorescence spectrum.The synchronous fluorescence intensity showed a good linear relationship with fermononetin concentration within the range of 0.005-1.28 μg·m L^-1.The regression equation was found to be IF=252.55462 c+4.95309,r=0.9996.Conclusion This method shows a high accuracy,low detection limit and reliable results.It can be used for the determination of trace amounts of formononetin.
作者
张宝成
吴剑
秦亚东
金欣
ZHANG-Baocheng;WU Jian;QIN Yadong;JIN Xin(Anhui College of Traditional Chinese Medicine,Wuhu 241002 Anhui,China)
出处
《中药新药与临床药理》
CAS
CSCD
北大核心
2018年第1期67-70,共4页
Traditional Chinese Drug Research and Clinical Pharmacology
基金
安徽省高等教育振兴计划人才项目--高校优秀青年人才支持计划项目(皖教秘人[2014]181号)
安徽中医药高等专科学校自然科学研究重点项目(ZRKX1801)
关键词
黄芪
芒柄花素
含量测定
三维同步荧光
Astragali radix
formononetin
determination
three-dimensional synchronous fluorescence spectrum
