摘要
目的研究肉毒碱棕榈酰基转移酶1A (CPT1A)在肺癌转移中的调控作用。方法首先,利用实时定量PCR (qRT-PCR)与Western blot实验检测11对肺癌原发灶与转移灶组织中CPT1A分子的mRNA与蛋白表达,以明确CPT1A在肺癌转移过程中的表达是否发生改变;其次,用细胞划痕实验检测下调CPT1A表达对肺癌A549细胞迁移能力的影响;最后,用Transwell侵袭实验检测下调CPT1A表达对肺癌A549细胞侵袭能力的影响。结果肺癌转移灶组织中CPT1A表达在m RNA与蛋白水平均明显高于原发灶组织[mRNA水平:(1.00±0.14)vs (1.83±0.75);蛋白水平:(1.00±0.26) vs (2.25±0.63)],差异均有统计学意义(P<0.05);与对照组细胞相比,下调CPT1A表达可显著抑制肺癌细胞的迁移能力[siCtrl vs siCPT1A-1 vs siCPT1A-2=(1.00±0.12) vs (0.39±0.04) vs (0.38±0.03)],差异均有统计学意义(P<0.05);与对照组相比,下调CPT1A表达可显著抑制肺癌细胞的侵袭能力[siCtrl vs siCPT1A vs siCPT1A-2=(23.00±3.00) vs (12.00±2.00) vs (13.00±1.00)],差异均有统计学意义(P<0.05)。结论 CPT1A在肺癌转移过程中表达显著上调;CPT1A可同时促进肺癌细胞的迁移与侵袭能力,提示CPT1A是肺癌治疗潜在的分子靶标。
Objective To investigate the regulation of carnitine palmitoyltransferase 1A(CPT1A) in lung cancer metastasis. Methods Firstly, real-time quantitative PCR(qRT-PCR) and Western blot were used to detect the expression of CPT1 A mRNA and protein in 11 pairs of primary and metastatic tissues of lung cancer, and to determine whether the expression of CPT1 A in lung cancer metastasis changed. Secondly, scratch wound healing assay was used to detect the effect of down-regulation of CPT1 A expression on the migration ability of lung cancer cells(A549 cell line).Finally, Transwell invasion assay was used to evaluate the effect of down-regulating CPT1 A expression on the invasion ability of lung cancer A549 cells. Results The expression level of CPT1 A in lung cancer metastatic tissues was significantly higher than that in primary tissues: m RNA level:(1.00±0.14) vs(1.83±0.75);protein level:(1.00±0.26) vs(2.25±0.63);P<0.05. Compared with the control cells, down-regulation of CPT1 A expression significantly suppressed the migration ability of lung cancer cells: siCtrl vs siCPT1A-1 vs siCPT1A-2 =(1.00±0.12) vs(0.39±0.04) vs(0.38±0.03), P<0.05. Down-regulation of CPT1 A expression also significantly inhibited the invasion abilities of lung cancer cells compared with the control group: siCtrl vs siCPT1A-1 vs siCPT1A-2 =(23.00 ± 3.00) vs(12.00 ± 2.00) vs(13.00 ±1.00), P<0.05. Conclusion The expression levels of CPT1 A is significantly up-regulated during the metastasis of lung cancer. CPT1 A can simultaneously promote the migration and invasion of lung cancer cells, suggesting that CPT1 A is a potential molecular target for the treatment of lung cancer.
作者
贾建博
王涛
辛向兵
卢强
韩勇
田磊
JIA Jian-bo;WANG Tao;XIN Xiang-bing;LU Qiang;HAN Yong;TIAN Lei(Department of Thoracic Surgery,the Second Affiliated Hospital of Fourth Military Medical University, Xi'an 710038,Shaanxi,CHINA;Department of Cardiovascular Surgery,the First Affiliated Hospital of Fourth Military Medical University,Xi'an 710032,Shaanxi,CHINA)
出处
《海南医学》
CAS
2019年第1期5-8,共4页
Hainan Medical Journal
基金
陕西省自然科学基础研究计划重点项目(编号:2015JZ024)
关键词
肉毒碱棕榈酰基转移酶1A
脂肪酸氧化
迁移
侵袭
肺癌
Carnitine palmitoyltransferase 1A
Fatty acid oxidation
Migration
Invasion
Lung cancer
