摘要
目的探讨益气补血、活血解毒经验药方归芪白术方对顺铂所致小鼠肾脏损伤的减毒作用。方法 50只雄性昆明小鼠随机分为空白组、模型组及归芪白术方低、中、高剂量组(n=10)。模型组及归芪白术方低、中、高剂量组每7d注射一次顺铂(剂量2.0mg/kg,每次0.2ml),共注射4次。归芪白术方低、中、高剂量组每日17:00用归芪白术方灌胃,每次0.4ml;空白组与模型组每日17:00采用等量生理盐水灌胃。每2d测量一次小鼠体重,每4d测量一次饮食情况,末次注射顺铂后48h采血检测各组小鼠血常规及肾脏功能,剥离各组小鼠肾脏组织,观察肾脏大体形态变化并计算肾脏指数,检测肾脏病理变化及肾脏组织中caspase 3、caspase 9的表达变化。结果空白组小鼠体重和饮食量维持在一个较高的平均水平;模型组小鼠体重呈周期性地降低和恢复,饮食量则明显降低;给药(归芪白术方)后,小鼠体重和饮食的降低得到一定缓解。肾脏大体观可见空白组小鼠肾脏颜色深而富有血液;模型组小鼠肾脏颜色明显变浅,体积变小,肾脏指数明显降低;给药(归芪白术方)后,肾脏体积有所增加,颜色较模型组加深,肾脏指数升高,其中,中、高剂量组改善较明显,与模型组比较差异有统计学意义(P<0.05)。与空白组比较,模型组小鼠白细胞计数(WBC)、血小板计数(PLT)明显降低,尿素氮(BUN)、血肌酐(Scr)水平及caspase3、caspase9表达量明显升高,差异均有统计学意义(P<0.05);与模型组比较,归芪白术方低、中、高剂量组小鼠WBC、PLT明显升高,BUN、Scr水平降低,caspase 3、caspase 9表达量明显下调,以中、高剂量最为明显,差异有统计学意义(P<0.05),低剂量组Scr水平与模型组比较差异无统计学意义。病理形态学观察显示,空白组小鼠肾脏组织结构正常,无明显变性、坏死及炎性浸润,可见到清晰的肾小管结构;模型组小鼠肾小管结构浊肿,近曲小管上�
Objective To explore the attenuation effect of Guiqi Baizhu prescripion on kidney injury induced by cisplatin in mice. Methods Fifty male Kunming mice were randomly divided into 5 groups(10 each): blank group, model group, and low, medium, and high dose of Guiqi Baizhu prescripion group. Mice in the 4 latter groups were intraperitoneally injected with cisplatin(2.0mg/kg, 0.2ml) once every 7d for 4 times. Mice in the 3 latter groups were gavaged with Guiqi Baizhu prescripion(0.4ml) at 17:00 every day, and in blank group and model group were gavaged with equivalent normal saline at 17:00 every day. The weight of mice was detected every 2 days, and the appetite was detected every 4 days. Blood routine and renal function tests were performed 48 hours after the last cisplatin injection, and the kidney tissues of all the mice were stripped, the general morphological changes of kidney were observed, the kidney index was calculated, and the renal pathological changes and the expression of cysteinyl aspartate specific proteinase 3(caspase 3) and cysteinyl aspartate specific proteinase 9(caspase 9) in kidney tissue were also detected. Results The body weight and appetite of control group were maintained in a higher average level, while the body weight of model group showed cyclical decrease and recovery, and the appetite presented significant reduction. After administration of Guiqi Baizhu prescription, the reduction of body weight and appetite got some mitigation. As to the general morphology of the kidney, those in blank group were with deep color and rich blood, and in model group with apparently lighter colour, smaller volume, and significantly decreased kidney index. After administration of Guiqi Baizhu prescription, the kidney volume increased, color deepened, and kidney index increased compared with that in model group, and these changes were more obvious in medium and high dose group with significant difference compared to that in model group(P<0.05). As to the biochemical function, the white blood cell count(WBC)
作者
王磊
许小敏
张艳辉
刘永琦
张利英
张丽昕
张苡铭
何进鹏
危文俊
WANG Lei;XU Xiao-min;ZHANG Yan-hui;LIU Yong-qi;ZHANG Li-ying;ZHANG Li-xin;ZHANG Yi-ming;HE Jin-peng;WEI Wen-jun(Specialty of Integrated Chinese and Western Medicine,Gansu University of Traditional Chinese Medicine,Lanzhou 73000G China;Department of Oncology,Traditional Chinese Medicine Hospital of Yunyang Country,Yunyang,Chongqing 404500,China;Institute of Modern Physics,Chinese Academy of Sciences,Lanzhou 730000,China)
出处
《解放军医学杂志》
CAS
CSCD
北大核心
2018年第11期917-923,共7页
Medical Journal of Chinese People's Liberation Army
基金
甘肃中医药大学研究生创新基金(2017CX30).
关键词
归芪白术方
顺铂
肾脏损伤
Guiqi Baizhu prescription
cisplatin
renal injury
