摘要
目的探讨体外乏氧环境下低氧诱导因子-1α(hypoxia inducible factor-1α,HIF-1α)对诱导肝癌细胞逆向分化为肝癌干细胞并维持恶性生物学行为的影响。方法采用免疫磁珠分选出HepG2细胞中的CD133阴性细胞,分为2个大组:转染组转染siRNA-HIF-1α以沉默HIF-1α基因的表达,空白对照组不转染任何siRNA片段。2类细胞分别进行常氧及乏氧条件培养,本实验共分4组。采用MTT、克隆形成实验及Transwell小室实验检测细胞的增殖和侵袭能力,采用Western blot法及RT-PCR法检测细胞中HIF-1α、CD133、CD90及CD44的mRNA及其蛋白的表达。结果 MTT实验结果显示:4组细胞的增殖率随乏氧培养时间延长而增高;24 h及以后,与空白对照组相比,经siRNA-HIF-1α转染后,转染常氧组和乏氧组的细胞增殖率降低(P<0.05)。平板克隆实验结果显示:转染常氧组与转染乏氧组、空白对照常氧组与空白对照乏氧组比较其细胞形成克隆数的差异均有统计学意义(P<0.05)。Transwell小室实验结果显示:乏氧培养后,转染组与空白对照组相比,迁移至下室的细胞数目减少(P<0.05)。Western blot及RT-PCR结果显示:空白对照乏氧组中HIF-1α及肿瘤干细胞标志物(CD133、CD90、CD44)蛋白及其mRNA的表达水平均高于其余3组(P<0.05);经siRNA-HIF-1α转染后,转染乏氧组中HIF-1α及肿瘤干细胞标志物(CD133、CD90、CD44)蛋白及mRNA的表达水平均较转染常氧组和空白对照常氧组降低(P<0.05)。结论在乏氧环境下,低氧诱导因子HIF-1α可促进肝癌细胞逆向分化为肝癌干细胞并增强其恶性生物学行为。
Objective To explore the effects of hypoxia inducible factor-1 alpha(HIF-1α) on the reverse differentiation of hepatocellular carcinoma cells into liver cancer stem cells, and the maintenance of malignant biological behavior in hypoxic environment. Methods CD133-negative cells in HepG2 cells were separated by immunomagnetic beads and divided into two groups. The cells of siRNA group were transfected with siRNA-HIF-1α to silence the expression of HIF-1α gene, while cells of the blank control group did not transfect any siRNA fragments. The two groups of cells were cultured under normal and hypoxic conditions respectively. MTT, cloning and Transwell chamber experiments were used to detect the proliferation and invasion ability of cells. Western blot and real-time PCR(RT-PCR)were used to detect the expressions of HIF-1α, CD133, CD90, and CD44 protein and mRNA in cells. Results MTT results showed that the cell proliferation rate increased with the prolongation of hypoxia in four groups. Compared with the blank control group at 24, 32, 40, and 48 hours, the cell proliferation rate decreased significantly after siRNA-HIF-1a transfection, on both two kinds of cultured conditions(P<0.05). The results of plate cloning experiment showed that the number of cell-forming clones increased significantly after hypoxic culture(there were significant differences between the transfected normoxic group and transfected hypoxic group, blank control normoxic group and blank control hypoxic group, P<0.05);and the formation of transfected hypoxic condition group at the same time of hypoxia was also significant(P<0.05). The number of clones were significantly less than that of the blank control group at the hypoxic condition(P<0.05). Transwell lab experiment showed that after hypoxic culture, the number of cells migrated to the inferior chamber in the transfection group was significantly reduced compared with that of the blank control group(P<0.05).Western blot and RT-PCR results showed that the expression levels of HIF-1α protein an
作者
黄晶晶
卢乐
吉鸿
陆宏伟
HUANG Jingjing;LU Le;JI Hong;LU Hongwei(Department of General Surgery,The Second Affiliated Hospital of Xi'an liaotong University,Xi'an 710004,P.R.China;Department of General Surgery,Ankang People's Hospital,Ankang,Shaanxi 725000,P.R.China)
出处
《中国普外基础与临床杂志》
CAS
2019年第1期14-18,共5页
Chinese Journal of Bases and Clinics In General Surgery
基金
陕西省自然科学基础研究计划项目(项目编号:2014JM2-8189)
西安交通大学第二附属医院科研基金青年项目[项目编号:YJ(QN)201113]
关键词
人肝癌HEPG2细胞
肝癌干细胞
乏氧环境
低氧诱导因子-1Α
逆分化
hepatocellular carcinoma HepG2 cell
liver cancer stem cell
hypoxia
hypoxia inducible factor-1 alpha
reverse differentiation
