摘要
选择菹草(Potamogeton crispus)和凤眼蓝(Eichhornia crassipes),分别设置不同浓度(10μmol/L、20μmol/L、50μmol/L和100μmol/L、150μmol/L、200μmol/L)的外源3-吲哚乙酸(Indole-3-acetic acid,IAA),于2018年1月26日~2月3日,进行室内水培实验,研究其对Cu(NO3)2胁迫下菹草和凤眼蓝抗氧化酶活性和Cu2+含量的影响,分析了IAA产生作用的机理。研究结果表明,在Cu(NO3)2胁迫下,施加外源IAA能显著提高菹草和凤眼蓝的根长和叶绿素含量,菹草的根长和叶绿素含量最高分别为10.24 cm和8.96 mg/L,凤眼蓝的分别为7.65 cm和12.27 mg/L,但是降低了其丙二醛含量(n=6,p<0.05)。在Cu(NO3)2胁迫下,添加IAA使凤眼蓝和菹草的超氧化物歧化酶和过氧化物酶活性显著增强(n=6,p<0.05),在施加高浓度IAA条件下,菹草的过氧化氢酶活性却受到抑制,为14.45 U/mg。施加IAA后,凤眼蓝各部位和菹草根中的Cu2+含量显著增加(n=4,p<0.05),但是菹草茎叶中的Cu2+含量显著减少(n=4,p<0.05),且其在Cu(NO3)2胁迫+施加高浓度IAA条件下最低。在Cu(NO3)2胁迫+IAA条件下,施加中浓度IAA,使凤眼蓝和菹草的转运系数最小,分别为0.30和1.21。外源IAA通过提高两种植物的抗氧化酶活性和降低膜脂过氧化物含量,缓解了Cu(NO3)2胁迫对两种植物的毒害,增强了植物对Cu(NO3)2的耐受能力,凤眼蓝比菹草具有更强的抗氧化能力。
Taking Potamogeton crispus and Eichhornia crassipes as the experimental object,three different concentrations of exogenous indole-3-acetic acid(IAA)(10μmol/L,20μmol/L and 50μmol/L for Potamogeton crispus;100μmol/L,150μmol/L and 200μmol/L for Eichhornia crassipes)were set up for indoor hydroponic experiment from January 26 to February 3,2018,to study the antioxidant enzyme activity and copper contents of Potamogeton crispus and Eichhornia crassipes under Cu(NO3)2stress.The mechanism of indole-3-acetic acid production was analyzed.The results showed that under Cu(NO3)2stress,the application of exogenous indole-3-acetic acid significantly increased the root length and chlorophyll contents of Potamogeton crispus and Eichhornia crassipes,with the root length and chlorophyll contents of Potamogeton crispus reaching10.24 cm and 8.96 mg/L,respectively,and those of Eichhornia crassipes reaching 7.65 cm and 12.27 mg/L,respectively,while the content of malondialdehyde of both Potamogeton crispus and Eichhornia crassipes decreased(n=6,p<0.05).Under Cu(NO3)2stress,the addition of indole-3-acetic acid significantly increased activity of superoxide dismutase and peroxidase of Eichhornia crassipes and Potamogeton crispus(n=6,p<0.05),while the catalase activity of Potamogeton crispus was inhibited at a high concentration of 14.45 U/mg.After applying indole-3-acetic acid,Cu2+contents in various parts of Eichhornia crassipes and roots of Potamogeton crispus increased significantly(n=4,p<0.05),but Cu2+contents in stems and leaves of Potamogeton crispus decreased significantly(n=4,p<0.05),and reached the lowest at Cu(NO3)2stress and high concentration's indole-3-acetic acid.With Cu(NO3)2stress and medium concentration's indole-3-acetic acid,the transport coefficient of Eichhornia crassipes and Potamogeton crispus was the smallest,reaching 0.30 and 1.21,respectively.In conclusion,exogenous indole-3-acetic acid alleviates the toxicity of Cu(NO3)2stress to the two plants by increasing the activity of antioxidant enzymes and reduci
作者
郗厚叶
王森
李悦
李园园
孔范龙
XI Houye;WANG Sen;LI Yue;LI Yuanyuan;KONG Fanlong(College of Environmental Science and Engineering,Qingdao University,Qingdao 266071,Shandong,P.R.China)
出处
《湿地科学》
CSCD
北大核心
2018年第6期816-824,共9页
Wetland Science
基金
国家自然科学基金项目(41771098)资助
关键词
3-吲哚乙酸
硝酸铜胁迫
凤眼蓝
菹草
酶活性
indole-3-acetic acid
Cu(NO3)2 stress
Eichhornia crassipes
Potamogeton crispus
enzyme activity
