摘要
目的应用荧光原位杂交技术对一家系中染色体核型为15p+的患者进行分析。方法经细胞遗传学(G显带)发现染色体核型为15p+的患者选用Yq12为探针进行荧光原位杂交。结果 15号染色体短臂的额外物质为Y染色体长臂末端的结构异染色质。结论 FISH方法可帮助确定一些未知来源染色体片段。
Objective:To analyze the chromosome karyotype of 15 p + in a family by fluorescence in situ hybridization. Methods:Patients with karyotype 15 p+ were selected by cytogenetics(G-banding)for fluorescence in situ hybridization using Yq12 as a probe. Results:The extra material of the short arm of chromosome 15 is the structural heterochromatin at the end of the long arm of the Y chromosome. Conclusion:The FISH method can help identify chromosome fragments from unknown sources.
作者
芦清霞
孙雪梅
蒋露薇
李静
LU Qing-xia;SUN Xue-mei;JIANG Lu-wei;LI Jing(Nanjing Subbo Medical Cloud Biotechnology Co.,Ltd.Nanjing Laboratory;Xinjiang Jiayin Hospital (Limited)Genetics Room)
出处
《中国优生与遗传杂志》
2018年第12期62-62,59,F0002,共3页
Chinese Journal of Birth Health & Heredity
关键词
不孕不育
荧光原位杂交
染色体
易位
Fluorescence in silu hybridization
Chromosome
Translocation
