摘要
目的:对胃癌前病变(Precancerous Lesion of Gastric Cancer,PLGC)模型大鼠从整体、细胞及分子水平的指标进行观察,分析其胃黏膜微环境的特点,并初步探讨半夏泻心汤对其胃黏膜的作用机制。方法:清洁级雄性SD大鼠70只,随机分成3组:模型组50只,造模加中药干预组10只,空白组10只。采用改良MNNG+复合法制备PLGC大鼠模型,于造模中期第10周、造模结束期第20周,各组随机抽取5只大鼠,分别从整体水平、细胞水平及分子水平检测半夏泻心汤对其胃黏膜微环境的影响。结果:1)启动子PTEN,造模加中药干预组较模型组表达高,但仍低于空白组; 2)调控器PI3K/Akt/mTOR信号通路,PI3K、Akt、mTOR的表达造模加中药干预组较模型组低,但仍高于空白组; 3)效应子HIF-1α及其下游基因,造模加中药干预组较模型组表达低,但仍高于空白组;上述结果差异均有统计学意义(P <0. 05)。结论:中药半夏泻心汤通过影响PLGC大鼠胃黏膜组织微环境变化的3个关键环节,即PI3K/Akt/mTOR信号通路中的启动子、调控器及效应子,从而影响并阻断PLGC的发生发展。
Objective:To observe various indices of a rat model of precancerous lesion of gastric cancer(PLGC)at gross,cellular,and molecular levels,to analyze the characteristics of gastric mucosal microenvironment in the rats,and to primarily explore the mechanism of action of Banxia Xiexin decoction on gastric mucosa.Methods:A total of 70 clean male Sprague-Dawley rats were randomly divided into three groups:50 in model group,10 in modeling plus traditional Chinese medicine treatment group(MTCM group),and 10 in blank group.The rat model of PLGC was established by modified MNNG + composite method.At the 10th week of modeling(interim period)and the 20th week of modeling(end period), 5 rats were randomly selected from each group to determine the effect of Banxia Xiexin decoction on gastric mucosal microenvironment in the rats at gross,cellular,and molecular levels.Results:1)The MTCM group had a higher expression level of the promoter PTEN than the model group,but it was still lower than that in the blank group;2)The MTCM group had a lower expression level of the signal pathway regulators PI3K/Akt/mTOR than the model group,but it was still higher than that in the blank group;3)The MTCM group had a lower expression level of the effector HIF-1α and its downstream genes than the model group,but it was still higher than that in the blank group;all the differences in the above results were significant(P<0.05).Conclusion:The traditional Chinese medicine Banxia Xiexin decoction affects and prevents the development and progression of PLGC by involving three key steps in the microenvironmental changes of gastric mucosa in the rats with PLGC,namely promoters,regulators,and effectors in the PI3K/Akt/mTOR signaling pathway.
作者
刘嘉诚
刘洁
LIU Jiacheng;LIU Jie(Tianjin University of Traditional Chinese Medicine,Tianjin 300193,China;The Second Affiliated Hospital of Tianjin University of Traditional Chinese Medicine,Tianjin 300150,China)
出处
《湖南中医杂志》
2018年第12期117-119,共3页
Hunan Journal of Traditional Chinese Medicine
基金
天津市高等学校科技发展基金计划项目(编号:20140217)
