摘要
目的探讨肥胖在感染肺炎时对机体免疫功能的调节是否与脾脏和外周血中T淋巴细胞亚群的变化有关。方法将常规非肥胖小鼠分为Ⅰ、Ⅱ组,将高脂诱导小鼠分为Ⅲ、Ⅳ组,Ⅰ、Ⅲ组滴鼻40μl PBS溶液,Ⅱ、Ⅳ组滴鼻40μl含5.9×10^(10)CFU大肠杆菌的菌液,于感染前(0 h)和感染后12 h、24 h、72 h检测各小组脾脏和外周血中的T淋巴细胞亚群。结果在感染后12 h、72 h,Ⅳ组脾脏的CD3^+细胞比例较Ⅲ组显著下降(P<0.05),Ⅱ组CD4+细胞比例较Ⅰ组显著升高(P<0.05);在感染后24 h、72 h,Ⅳ组CD4^+细胞比例较Ⅲ组显著下降(P<0.05)。在感染后24 h、72 h,Ⅱ组CD8^+细胞比例组较Ⅰ组显著下降(P<0.05);在感染后12 h、24 h、72 h,Ⅳ组CD8^+细胞比例较Ⅲ组显著下降(P<0.05)。在感染后12 h、24 h、72 h,Ⅱ组CD4^+/CD8^+比值较Ⅰ组显著升高(P<0.05)。各组外周血中的CD3^+细胞比例,在感染后12 h、72 h,Ⅳ组显著高于Ⅱ组(P<0.05);各组外周血中的CD4+细胞比例,在感染前(0 h),Ⅲ组显著高于Ⅰ组,Ⅳ组显著高于Ⅱ组(P<0.05)。结论感染非致死性肺炎后,肥胖机体能动员脾脏更多的T淋巴细胞释放入血,加强了机体的细胞免疫功能,有利于抵抗外来病原体的入侵。
This study was designed to investigate the impact of obesity on T lymphocyte subsets in mice infected with nonfatal pneumonia. The mice fed with standard diet were divided into groups Ⅰand Ⅱ, while high-fat diet induced obese mice were divided into groups Ⅲ and Ⅳ. Mice in groups Ⅰ and Ⅲ were given 40 μl fluid of phosphate buffer saline(PBS), while mice in groups Ⅱ and Ⅳ were intranasally infused with 40 μl fluid containing5.9×10^10 CFU Escherichia coli(E. coli). T lymphocyte subsets of all groups were detected at 0 h, 12 h, 24 h, 72 h postinfection. Data showed that in spleen, the percentage of CD3^+ cells in group Ⅳ was significantly decreased at 12 h and 72 h post-infection, as compared with group Ⅲ; the percentage of CD4+cells in group Ⅱ was significantly increased at 12 h and 72 h post-infection, as compared with group Ⅰ; the percentage of CD4^+ cells in group Ⅳ was significantly decreased at 24 h and 72 h post-infection, as compared with group Ⅲ; the percentage of CD8^+ cells in group Ⅱ was significantly lower than that in group Ⅰ at 24 h and 72 h post-infection; the percentage of CD8+cells in group Ⅳ was significantly lower than that in group Ⅲ at 12 h, 24 h, 72 h post-infection; the ratio of CD4^+/CD8^+ in group Ⅱ was significantly increased at 12 h, 24 h, 72 h post-infection, as compared with group Ⅰ. In peripheral blood, at 0 h post-infection(before infection), the percentage of CD4+cells in group Ⅲ and group Ⅳ were significantly higher than those in groupⅠ and group Ⅱ, respectively. After infection, the percentage of CD3^+ cells in group Ⅳwas significantly higher than that in group Ⅱ at 12 h and 72 h post-infection.The results revealed that obesity can mobilize more T cells of spleen to release into blood, which enhances the cellular immune function, and is helpful to resist the invasion of foreign pathogens.
作者
梁爽
左之才
辜旭初
方静
刘晓
王宇
阳明贤
LIANG Shuang;ZUO Zhicai;GU Xuchu;FANG Jing;LIU Xiao;WANG Yu;YANG Mingxian(Key Laboratory of Animal Disease and Human Health of Sichuan Province,College of Veterinary Medicine,Sichuan Agricultural University,Chengdu 611130,China)
出处
《免疫学杂志》
CAS
CSCD
北大核心
2019年第1期21-28,共8页
Immunological Journal
基金
国家重点研发计划(2018YFD0501800)
四川省科技计划(2018NZ002)
国家现代农业产业技术体系四川肉牛创新团队(035Z2388)
关键词
肥胖
非致死性肺炎
脾脏
外周血
T淋巴细胞亚群
Obesity
Non-fatal pneumonia
Spleen
Peripheral blood
T lymphocyte subsets
