摘要
为了获得黑松Pinus thunbergii-美味牛肝菌Boletus edulis(Be)菌塘中的菌根促生菌(mycorrhiza helper bacteria,MHB),为美味牛肝菌的人工培育提供理论基础,通过比较野生牛肝菌菌塘细菌胞外代谢产物对菌丝生长的促进作用以及细菌+Be真菌双接处理黑松幼苗对其苗高、地径、茎根比、干重、根侵染率的影响,筛选出菌根促生菌。结果表明,菌株B2和K2代谢产物可以显著促进Be菌丝生长,盆栽实验结果表明双接种Be+B2、Be+K2的黑松苗,苗高分别增长了17.71%和16.82%,地径增长了41.65%和35.63%,干重提高了44.71%和48.78%,根侵染率提高了23.20%和21.50%,茎根比下降了46.41%和35.40%。可见菌株B2和K2是黑松-美味牛肝菌的菌根促生菌。通过细菌的形态、生化特性、16SrDNA序列测定,菌株B2和K2均鉴定为蜡样芽孢杆菌Bacillus cereus。蜡样芽孢杆菌有望进一步开发为美味牛肝菌的菌塘生物肥料。
Mycorrhiza helper bacteria(MHB)in rhizosphere soil of Pinus thunbergii and Boletus edulis(Be),were isolated,and their positive effect on growth of ectomycorrhizal fungus Be was investigated.The effect of Be and MHB co-inoculation on pine seedling height,ground diameter of stem,stem root ratio,seeding dry weight and root infection rate was also observed.The result revealed that the metabolites of bacterial strains B2 and K2 significantly promoted in vitro hyphal growth of Be.Co-inoculation of Be+B2 and Be+K2 in pot experiment showed that stem root ratio decreased by 46.41% and 35.40% respectively.However,seedling height,ground diameter,dry weight,root infection rate increased by 17.71% and 16.82%,41.65% and 35.6%,44.71% and 48.78%,and 23.2% and 21.5%,respectively.It was concluded that B2 and K2 can be considered as MHB strains.B2 and K2 were identified as Bacillus cereus based on morphological,physiological,and biochemical analyses in combination with analysis of 16S rDNA gene sequences.Bacillus cereus is expected to be developed as biological fertilizer in colonized soil of Boletus edulis.
作者
郭霞
郑雨焕
GUO Xia;ZHENG Yu-Huan(School of Life Sciences, Chongqing Normal University, Chongqing 401331, China)
出处
《菌物学报》
CAS
CSCD
北大核心
2018年第12期1802-1807,共6页
Mycosystema
基金
重庆师范大学基金项目(14XYY021)~~
关键词
美味牛肝菌
黑松
菌根促生细菌
蜡样芽孢杆菌
Boletus edulis
Pinus thunbergii
mycorrhiza helper bacterium
Bacillus cereus
