摘要
采用柱层析法对蛋黄卵磷脂纯化工艺进行研究。结果表明,从3种吸附介质中筛选出AB-8大孔吸附树脂为蛋黄卵磷脂的纯化树脂,并通过静态试验得到其对蛋黄卵磷脂的最大吸附率为92. 2%,最佳静态吸附时间是60 min。经过中压制备色谱分离,采用干法上样,用体积分数95%的乙醇洗脱,可得到蛋黄卵磷脂的纯化倍数为4倍,纯度达到83. 1%,回收率为97. 04%。AB-8大孔吸附树脂使用50个操作周期时,卵磷脂吸附率仍在80%以上。表明AB-8大孔吸附树脂纯化蛋黄卵磷脂具有纯化倍数高、回收率高、时间短、重复性好等优点。
The purification process of egg yolk lecithin by column chromatography was studied.The results showed that AB -8 macroporous adsorption resin was selected from three kinds of adsorbents to purify egg yolk lecithin ,.the maximum adsorption rate was 92.2%,and the optimal static adsorption time was 60min by static test.After separation by medium pressure preparation chromatography,using dry sample,eluted with 95% ethanol,purification multiple,purity and recovery rate of egg yolk lecithin were four times,83.1% and 97.04%,respectively.The lecithin adsorption rate was above 80% when AB -8 macroporous adsorption resin was used for 50 cycles of operation.Purification process of egg yolk lecithin with AB -8macroporous adsorption resin had advantages of high purification multiple,high recovery rate,short time,and good repeatability.
作者
吴昊
周旋
张椿
WU Hao;ZHOU Xuan;ZHANG Chun(Chemical Engineering Institute,Xuzhou College of Industrial and Vocational Technology,Xuzhou 221140,Jiangsu,China;School of Chemical Engineering &Technology,China University of Mining and Technology,Xuzhou 221116,Jiangsu,China)
出处
《中国油脂》
CAS
CSCD
北大核心
2018年第12期79-83,共5页
China Oils and Fats
基金
江苏省大学生创新创业训练计划项目(201713107006Y)
徐州市生化资源综合利用与清洁生产重点实验室开放课题(XGY20160406010)
江苏省高职院校教师专业带头人高端研修项目(2017GRFX053)
关键词
卵磷脂
分离纯化
大孔吸附树脂
中压制备色谱
lecithin
separation and purification
microporous adsorption resin
medium pressure preparation chromatography
