摘要
以蛹虫草为材料,采用响应面分析法对蛹虫草蛋白超声波辅助提取工艺参数进行优化,并对所提取蛹虫草蛋白氨基酸组成与营养价值进行分析。蛹虫草蛋白超声波辅助提取的最优条件为:料液比1∶50、pH=8.57,150 W功率下超声30 min,然后关闭超声波,置于35℃条件下继续搅拌提取72 min,此条件下蛋白质提取率达到85.6%,是不超声对照组蛋白提取率的1.68倍,与模型预测值相对误差为±0.77%。在最优工艺条件下所提取的蛹虫草蛋白含必需氨基酸占总氨基酸的比值为44.4%,必需氨基酸与非必需氨基酸的比值为79.84%,均大于世界粮食与农业组织和世界卫生组织的推荐值,表明所提蛹虫草蛋白质具有较高的营养价值,为蛹虫草蛋白的高效提取及蛹虫草的保健食品开发提供了参考。
The method of ultrasonic-assisted extraction of proteins from Cordyceps militaris were optimized by response surfacemethodology,and the amino acid composition and nutritional value of the extracted C. militaris proteins were analyzed. The optimal conditionsfor the ultrasonic-assisted extraction of proteins were as follows :solid-liquid ratio of 1∶50,pH of 8.57,and ultrasonic at 150 W for 30 min,then the ultrasonic wave was turned off,the mixture was further stirred at 35℃ and extraction was conducted for 72 min. Under theseconditions,the extraction rate of proteins reached 85.6%,which was 1.68 times of the non-ultrasound control group,and the relative errorwith the model prediction value was ±0.77%. Under the optimal conditions,the ratio of essential amino acids to total amino acids of C. militarisproteins was 44.4%,and the ratio of essential amino acids to non-essential amino acids was 79.84%,which was higher than the recommendedvalues by the World Food and Agriculture Organization(FAO)and the World Health Organization(WHO). It indicates that the extractedC. militaris proteins had high nutritional value,providing a reference for the efficient extraction of C. militaris proteins and the development ofhealth food from C. militaris.
作者
侯若琳
刘鑫
项凯凯
陈磊
郑明锋
傅俊生
HOU Ruo-lin;LIU Xin;XIANG Kai-kai;CHEN Lei;ZHENG Ming-feng;FU Jun-sheng(College of Food Science,Fujian Agriculture and Forestry University,Fuzhou 35002;College of Life Sciences,Fujian Agriculture and Forestry University,Fuzhou 35002)
出处
《生物技术通报》
CAS
CSCD
北大核心
2018年第11期198-204,共7页
Biotechnology Bulletin
基金
国家自然科学基金项目(81503187)
福建省重大农技推广服务试点项目(KNJ-153011-4)
福建农林大学现代种业工程研究院建设经费
关键词
蛹虫草
蛋白
超声波辅助
响应面
氨基酸分析
Cordyceps militaris
protein
ultrasonic-assisted
response surface
amino acid analysis
