摘要
目的:比较5种常用复方中药注射液和粒细胞集落刺激因子(G-CSF)治疗化疗相关白细胞减少症小鼠的疗效,以及对小鼠多脏器及胸腺的影响。方法:采用环磷酰胺腹腔注射法制备小鼠化疗相关白细胞减少症模型,以G-CSF为阳性对照,用5种复方中药注射液(康艾、复方苦参、参附、参麦和生脉注射液)对各治疗组小鼠分别腹腔注射给药,隔天抽取外周血测血常规,随后处死小鼠,取心脏血测血清肝、肾功能指标(ALT,AST,BUN和CR),取重要脏器(心、肝、脾、肺、肾)及胸腺计算相应脏器指数,并比较各组病理变化,以评价各治疗组的疗效和对多脏器及胸腺的影响。结果:5个中药治疗组小鼠的白细胞计数较模型组显著上升(P <0. 01),但低于阳性对照组(G-CSF)(P <0. 05,P <0. 01)。此外,5种复方中药注射液和G-CSF均有不同程度的升血小板作用(P <0. 05,P <0. 01)。与模型组相比,苦参治疗组(P=0. 036)、参附治疗组(P=0. 008)的血清AST略升高;各中药治疗组的血清ALT和肾功能指标未见明显升高,但是,脾脏指数下降(P <0. 05或P <0. 01),肺脏指数增加(P <0. 01)。各中药治疗组小鼠肝细胞可见不同程度的细胞变性、点状坏死和炎症反应,但组间差别不明显。除苦参治疗组外,其余中药治疗组的肝脏病理评分均显著低于模型组(P <0. 05,P <0. 01),其中生脉治疗组最明显(P <0. 01)。结论:5种中药注射液均能有效治疗化疗相关白细胞减少症,其作用弱于G-CSF。5种中药注射液和G-CSF还有一定升血小板作用。除苦参外,其余4种中药注射液可能有一定护肝作用。5种中药注射液对其他脏器和胸腺的影响不明显。
AIM: To investigate the effects of 5 commonly-used compound traditional Chinese medicine( TCM) injections and granulocyte-colony stimulating factor( G-CSF) in the treatment of chemotherapy-related leukopenia mice,as well as their effects on multiple organs and thymus of the mice.METHODS: Cyclophosphamide intraperitoneal injection was used to prepare mice model with Chemotherapy-related leukopenia. The mice treated with G-CSF were taken as positive control, while the mice in each treatment group were given intraperitoneal injection of kang ’ai,kushen,shenfu,shenmai and shengmai,respectively. The mice were killed the next day after drawing peripheral blood for routine blood test. Cardiac blood was taken to measure serum liver and kidney function indexes( ALT,AST, BUN and CR), while important organs( heart,liver,spleen,lung,kidney) and thymus were taken to calculate the corresponding organ index. RESULTS: Compared with the model group,the white blood cell( WBC) count of the mice in the five TCM-treated groups was significantly increased( P < 0. 01),which was lower than that of the GCSF-treated group( P < 0. 05,P < 0. 01). In addition,G-CSF and 5 kinds of TCM injections can promote platelet at different levels( P < 0. 05,P <0. 01). Compared with the model group,the level of serum AST was slightly increased in the kushen-treated group( P = 0. 036) and the shenfu-treated group( P = 0. 008). Serum ALT and renal function index were not significantly increased in each TCM-treated group,while the spleen index decreased( P < 0. 05 or P < 0. 01) and the lung index increased( P <0. 01). In 5 TCM-treated groups,the liver cells of the mice showed different degrees of cellular degeneration, spot-like necrosis and inflammatory response,but the difference between the groups was not significant. Expect for the kushen-treated group,the liver pathology score of the other TCM-treated groups was significantly lower than that of the model group( P < 0. 05,P < 0. 01),among which the most significant was in the shengmai-treated group( P
作者
张喜平
张翔
杨红健
邹德宏
乔恩奇
王准
何向明
俞星飞
章颖
唐镔镔
ZHANG Xiping;ZHANG Xiang;YANG Hongjian;ZOU Dehong;QIAO Enqi;WANG Zhun;HE Xiangming;YU Xingfei;ZHANG Ying;TANG Binbin(Department of Breast Surgery,Zhejiang Cancer Hospital,Hangzhou 310022,Zhejiang,China;Department of Hematology,the First Affiliated Hospital of Medical College of Zhejiang University,Hangzhou 310006,Zhejiang,China;Department of Radiotherapy,Zhefiang Cancer Hospital,Hangzhou 310022,Zhejiang,China;Department of Breast Surgery,Fudan University Shanghai Cancer Center,Shanghai 200032,China;Second Outpatient Department of Traditional Chinese Internal Medicine,Tongde Hospital of Zhejiang Province,Hang-zhou 310012,Zhefiang,China)
出处
《中国临床药理学与治疗学》
CAS
CSCD
2018年第10期1116-1125,共10页
Chinese Journal of Clinical Pharmacology and Therapeutics
基金
浙江省中医药科技计划项目(2015ZB021)
