摘要
在形态分类的基础上,采用多重PCR(Multiplex Species-Specific PCR)和DNA序列分析,基于细胞色素氧化酶Ⅰ(cytochromeoxidaseI,COI)基因构建系统进化树鉴定牡蛎种类及确定其分类地位,研究了广东雷州半岛东部沿岸牡蛎科(Ostreidae)常见牡蛎的种类及分布。本次研究从雷州半岛东部沿岸的11个地点采集了363个野生牡蛎样品,并对全部样品进行了形态分析和多重PCR的鉴定,对未鉴定出来的114个样品进行COI基因的扩增并测序,构建系统进化树进行种类分析,共检测出3属5种牡蛎。巨蛎属(Crassostrea)有两种,分别为熊本牡蛎(C.sikamea)185个和福建牡蛎(C.gigas angulata)109个;小蛎属(Saccostrea)有两种,棘刺牡蛎(S.echinata)48个和团聚牡蛎(S.malabonensis)19个,牡蛎属未定种Ostreasp.1有2个。结果表明,雷州半岛东部沿岸牡蛎种类丰富,至少有5种牡蛎分布于潮间带,其中优势种为熊本牡蛎和福建牡蛎,分布范围比较广。小蛎属和牡蛎属牡蛎数量较少,分布比较集中。
We used multiplex species-specific PCR and DNA barcoding to identified oysters in the eastern coast of Leizhou Peninsula,Guangdong,China.A total of 363 oysters were collected from 11 sites.All the oysters were identified by multiplex species-specific PCR and 114 oysters were identified in mitochondrial COI gene-based analysis.At least five oyster species were recognized.Two Crassostrea species were dominated and widely distributed:C.sikamea (185 samples) and C.gigas angulata (109 samples);two Saccostrea species:S.echinata (48 samples)and S.malabonensis (19 samples), limited in certain places,and 2samples belonged to genus Ostrea sp.1.
作者
崔宗梅
黄津伟
王海艳
胡利莎
刘玉盟
CUI Zong-Mei;HUANG Jin-Wei;WANG Hai-Yan;HU Li-Sha;LIU Yu-Meng(Institute of Oceanology,Chinese Academy of Sciences,Qingdao 266071,China;University of Chinese Academy of Sciences,Beijing 100049,China;Tianjin Normal University,Tianjin 300387,China)
出处
《海洋与湖沼》
CAS
CSCD
北大核心
2018年第6期1350-1357,共8页
Oceanologia Et Limnologia Sinica
基金
国家自然科学基金项目
41776179号
40406032号
40876084号
现代农业产业技术体系建设专项资金
CARS-47号
中国科学院战略性先导科技专项
XDA10020305号
国家科技基础性工作专项
2014FY110500号
大学生创新创业训练计划项目
201710065032号
关键词
雷州半岛
牡蛎科
多重PCR
COI
Leizhou Peninsula
Ostreidae
multiplex species-specific PCR
COI
