摘要
目的探讨龙血竭总黄酮(Sanguis Draconis flavones,SDF)对D-半乳糖致衰老大鼠抗氧化及免疫调节作用。方法将60只SD大鼠随机分为6组,分别为正常组、模型组、阳性组,SDF低、中、高剂量组。颈背部皮下注射5%D-半乳糖生理盐水溶液建立大鼠亚急性衰老模型,同时灌胃给予相应药物6周,给药期间每周称量1次大鼠体重;实验结束后测定大鼠胸腺和脾脏指数;同时测定大鼠血清中丙二醛(MDA)、蛋白质羰基(PC)、晚期糖基化终末产物(AGES)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-PX)的含量或活性;酶联免疫法(ELISA)测定脑组织中脂褐素(LF)、过氧化脂质(LPO)以及B型单胺氧化酶(MAO-B)含量或活性;透射电镜观察脑组织超微结构改变。结果大鼠亚急性衰老模型复制成功,与模型组比较,SDF高、中剂量组能够明显升高大鼠胸腺、脾脏指数; SDF高剂量组还可明显降低大鼠血清中MDA、PC、AGES的含量并升高SOD、GSH-PX的活性; SDF高、中剂量组能够降低大鼠脑组织中LPO、LF含量及MAO-B的活性;同时SDF高、中剂量组可改善大鼠脑组织中神经元的退行性改变。结论 SDF具有较好的延缓衰老作用,其机制可能与抗氧化及影响神经介质有关。
OBJECTIVE To investigate the antioxidant ability and immune adjustment function of Sanguis Draconis flavones (SDF)on D-galactose aging in rats.METHODS The healthy SD rats were randomly divided into 6 groups :blank control group,aging model group,positive control group,low-dose,medium-dose and high-dose of SDF groups.Aging model was induced by subcutaneously injecting rats with 5%D-galactose.Rats of each group received daily administration by gavage for 6 weeks and the rats were weighted once per week in experimental period.The thymus and spleen index were measured ;meanwhile,the contents of MDA,PC, AGES and the activity of SOD,GSH-PX in serum were detected after the experiment.The contents of LF,LPO and MAO-B activity in brain tissue were detected by ELISA method;the ultrastructural changes of brain tissue were observed by transmission electron microscopy.RESULTS Compared with the normal group,rat subacute aging model was replicated successfully;high-dose and mediumdose of SDF can significantly increase the thymus and spleen index of rats.high-dose of SDF can significantly decrease the contents of MDA,PC,AGES and increase the activity of SOD,GSH-PX;high-dose and medium-dose of SDF can reduce the LF,LPO contents and MAO-B activity in brain tissue.In addition,high-dose and medium-dose of SDF can improve the degeneration of neurons in rat brain.CONCLUSION SDF has obvious anti-aging effect on aging rats,which may be related to the antioxidant and the influence of neurotransmitters.
作者
李睿
贾德云
贾会玉
徐庆华
许冬瑞
罗胜勇
LI Rui;JIA De-yun;JIA Hui-yu;XU Qing-hua;XU Dong-rui;LUO Sheng-yong(Anhui Academy of Medical Sciences,Hefei 230061,China;School of lnternational Education,Anhui Xinhua University,Hefei 230061,China)
出处
《中国药学杂志》
CAS
CSCD
北大核心
2018年第23期2008-2013,共6页
Chinese Pharmaceutical Journal
基金
安徽省自然科学基金面上项目资助(1608085MH161)
关键词
龙血竭总黄酮
衰老
抗氧化
神经元退行性改变
Sanguis Draconis flavones
aging
antioxidation
neurodegenerative changes
