摘要
目的分析口腔鳞癌(oral squamous cell carcinoma,OSCC)中受体型蛋白酪氨酸磷酸酶K(protein tyrosine phosphatase,receptor type K,PTPRK)基因的甲基化状态及其mRNA和蛋白表达,探讨OSCC的发生、发展机制。方法收集OSCC组织57例及癌旁正常口腔黏膜组织41例,采用甲基化特异性PCR(methylation specific PCR,MSP)、RT-qPCR和免疫荧光染色分析OSCC组织及癌旁正常口腔黏膜组织中PTPRK基因甲基化状态、mRNA和蛋白的表达以及甲基化与临床病理特征之间的关系。结果 OSCC组织中PTPRK基因甲基化阳性率高于癌旁正常口腔黏膜(59. 65%vs 34. 15%,P <0. 05)。OSCC组织中PTPRK的mRNA和蛋白表达量明显低于癌旁正常口腔黏膜(mRNA水平:0. 53±0. 23 vs 1. 05±0. 08,P <0. 001;蛋白水平:0. 43±0. 21 vs 1. 02±0. 78,P <0. 01),OSCC组织中PTPRK基因mRNA的表达与PTPRK基因的甲基化状态相关(P <0. 01)。PTPRK基因的高甲基化状态与淋巴结转移、TNM分期及病理分级相关(P <0. 05)。结论 PTPRK基因启动子区Cp G岛高甲基化可能与OSCC的发生、进展相关,有可能成为OSCC早期诊断及药物治疗的分子靶点。
Purpose To investigate the methylation status,mRNA and expression of protein tyrosine phosphatase,receptor type K( PTPRK) gene in oral squamous cell carcinoma( OSCC),and to discuss the mechanism of its occurrence and development. Methods PTPRK methylation and its expression in oral tissues from 57 cases of OSCC and 41 cases of normal oral mucosa were detected by MSP,RT-qPCR and immunofluorescence staining. The relationship between methylation and clinicopathological parameters were analyzed. Results The methylation rate of PTPRK gene in OSCC was higher than that of normal oral mucosa( 59. 65% vs 34. 15%,P < 0. 05). The mRNA and protein expression of PTPRK in OSCC tissues was significantly lower than those of normal oral mucosa tissues( mRNA level: 0. 53 ± 0. 23 vs 1. 05 ± 0. 08,P < 0. 001. Protein level:0. 43 ± 0. 21 vs 1. 02 ± 0. 78,P < 0. 01). The mRNA expression of PTPRK in OSCC was correlated with methylation status of PTPRK( P < 0. 01). Hypermethylation status of PTPRK was related to lymph node metastasis,TNM stage and pathological grade( P < 0. 05). Conclusion PTPRK promoter hypermethylation may be related to the pathogenesis and progression of OSCC,which may be a molecular target for early diagnosis and drug therapy of OSCC.
作者
彭宏峰
王元杰
田松波
马冬
董伟
梁永强
耿菲
李金源
PENG Hong-feng;WANG Yuan-jie;TIAN Song-bo;MA Dong;DONG Wei;LIANG Yong-qiang;GENG Fei;LI Jin-yuan(School of Stomatology,Tangshan 063210,China;Department of Stomatology,Tangshan Vocational and Technical College,Tangshan 063300,China;Department of Oral Medicine,the Second Hospital of Hebei Medical University,Shijiazhuang 050017,China;School of Public Health,Tangshan 063210,China;School of Basic Medical Sciences,North China University of Science and Technology,Tangshan 063210,China)
出处
《临床与实验病理学杂志》
CAS
CSCD
北大核心
2018年第11期1235-1239,共5页
Chinese Journal of Clinical and Experimental Pathology
