摘要
目的 :探讨治疗剂量阿霉素 (ADR)对心脏血流动力学及心肌肌浆网 Ca2 + - ATP酶活性的影响。方法 :实验兔每周静脉注射 ADR(2 mg/ kg) 1次 ,共 8周。以健康同龄兔静脉注射等量生理盐水为对照组。停药 3周后多普勒测定降主动脉血流量 ,以代表心输出量 (CO)。左颈总动脉和左心室插管测定血压 (BP) ,平均动脉压 (MAP) ,左心室收缩压 (LVSP) ,左心室舒张末压 (LVEDP)。应用荧光分光光度计测定心肌细胞内游离钙 (Myo Ca2 + )浓度 ,无机磷酸根法测定心肌肌浆网 (SR) Ca2 + - ATP酶活性。结果 :阿霉素组 CO、BP、MAP、LVSP均较对照组低 ,而LVEDP则明显增高。阿霉素组使 Myo Ca2 + 浓度显著增高 ,同时 SR Ca2 + - ATP酶活性明显低于对照组。结论 :治疗剂量阿霉素可使心脏功能下降 ,心肌细胞内钙超载及 SR Ca2 + -
Objective: To evaluate influences of regular dose of adriamycin (ADR) on heart function and sarcoplasmic reticulum (SR) Ca 2+ ATPase in cardiomyocyte of rabbits. Methods: Nine rabbits received intraveneous injection of ADR (2 mg/kg) once a week for 8 weeks,the rabbits injected with saline were used as control group.Cardiac output (CO),blood pressure (BP),mean artery pressure (MAP),left ventricular systolic pressure (LVSP),left ventricular end diastolic pressure (LVEDP), calcium in cardiomyocyte (MyoCa 2+ ) of rabbits and SR Ca 2+ ATPase activity were examinated 3 weeks after the final injection. Results: CO,LVSP and SR Ca 2+ ATPase activity were significantly decreased in ADR treated group compared with the control group.Conversely,LVEDP and MyoCa 2+ were significantly increased in ADR treated rabbits.Conclusion: Heart function can be decreased by regular dose of ADR injection.Calcium overload in cardiomyocyte and decrease of SR Ca 2+ ATPase activity is important physiopathologic mechanism in ADR induced impairment of heart.
出处
《浙江大学学报（医学版）》
CAS
CSCD
2002年第1期37-40,共4页
Journal of Zhejiang University(Medical Sciences)
