摘要
通过单因子试验统计分析 ,优化筛选了适于金针菇 (Flammulinavelutipes)FV90 8的适宜培养基和摇瓶培养条件 ,结果表明 ,其适宜的液体培养基组成为玉米粉 5 .0 % ,麸皮 2 .0 % ,KH2 PO4 0 .1% ,MgSO4 ·7H2 O 0 .0 5 % ,10 μgVB1/ 10 0mL ,5 0 μgVB2 / 10 0mL ;适宜的摇瓶培养条件为 :培养基的起始pH 6 .0～ 7.0 ,5 0 0mL摇瓶装量为15 0mL ,接种量为 10 % ,培养温度 2 5℃ ,摇床转速为 12 0r/min ,菌丝干收率 39g/L。
A suitable liquid medium for shaking culture of Flammulina velutipes FV908 was screened using single test statistics. Composition of the medium consists of 5.0% cornmeal, 2.0% wheat bran, 0.1% KH 2PO 4, 0.05% MgSO 4·7H 2O, 10μgVB-1/100mL, and 50μgVB-2/100mL. Under condition of pH 6.0～7.0,10% of inoculation, 25℃, 120 r/min, 150 mL medium in 500mL flask, the recovered dried mycelium was 4.1g/100 mL.
出处
《微生物学杂志》
CAS
CSCD
2002年第1期18-19,共2页
Journal of Microbiology
