摘要
U937是一种前单核细胞 ,其主要是以悬浮生长为主 ,细胞形态成圆球状 ,表面光滑 ,没有突起。在 PMA、内毒素或生长因子的刺激下细胞可由悬浮状态衍变为贴壁生长 ,细胞形态由圆球状变化为多边型 ,细胞表面有突起伸出。本实验采用流式细胞术检测了 U937与鼻咽癌细胞 CNE— 2混合培养对其 CD36表达的影响。结果发现 :U937与CNE— 2混合培养以促进 U937对 CD36的表达 ,共同培养 2 4小时 ,CD36表达显著增加。表明
U937 is a kind of pro monocyte.It grows mainly in the state of suspension. The shape of the cells are round and without processes. When stimulated with PMA, LPS or growth factor,the cells adhere to the culture plate and show processes.In present study U937 was co cultured with CNE-2 for 24 h and the expression of CD36 was detected by flow cytometry. The results showed that the co culture could promote U937 adhesion to CNE-2 and the expression of CD36 increased significantly.It means that the co culture can promote the differentiation of U937 the pro monocyte.
出处
《四川解剖学杂志》
2000年第2期65-69,共5页
Sichuan Journal of Anatomy
