摘要
背景与目的:端粒酶在女性生殖道发生的上皮性肿瘤中的检测已有相关报道,但对于卵巢性索-间质瘤中端粒酶活性的研究尚未见报道,本研究旨在探讨端粒酶活性在卵巢性索-间质肿瘤中的表达状况及其临床病理学意义。方法:收集25例卵巢性索-间质肿瘤,包括粒层细胞瘤、卵泡膜纤维细胞瘤、硬化性间质瘤及支持-间质细胞瘤。采用免疫组化SLAB法,以多克隆抗体H231检测人端粒酶反转录酶(humantelomerasereversetranscriptase,hTRT)在石蜡包埋肿瘤组织中的表达。结果:hTRT蛋白主要定位于瘤细胞(特别是黄素化或/和间质细胞)胞浆,少部分胞核同时表达。hTRT总阳性率为60.0%,不同类型肿瘤阳性表达分别为:硬化性间质瘤4/4、支持-间质细胞瘤5/5、粒层细胞瘤6/9,卵泡膜纤维细胞瘤0/7。出现内分泌功能紊乱症状者16例,12例表达hTRT,占75.0%。无内分泌症状者则呈阴性或弱阳性。hTRT阳性率与肿瘤类型和内分泌功能紊乱有关(P值分别为0.01和0.041),但与肿瘤分化程度及患者预后无关(P值均>0.05)。结论:在卵巢性索-间质肿瘤中,向黄素化细胞和间质细胞分化的瘤细胞端粒酶活性较高,内分泌功能活跃的细胞其端粒酶活性也可能增强,从而导致患者出现内分泌功能紊乱的症状。
Background & Objective: Telomerase activity has been detected in a broad range of human cancers including epithelial malignancies from in female genital tract, but the expression of the telomerase activity in ovarian sex cord tumors has not been reported up to now. In this report we investigated the telomerase activity in ovarian sex cord stromal tumors and its relationship with clinicopathological feature. Methods: Twenty five cases of ovarian sex cord stromal tumor, including granulosa cell tumors, thecofibromas, sclerosing stromal tumors, and Sertoli Leydig cell tumors were retrieved, and the expression of human telomerase reverse transcriptase (hTRT) was assessed immunohistochemically using polyclonal antibody H231. Results: hTRT were positive in 60.0% of all cases, and the signal mainly located in the cytoplasm of tumor cells, especially in luteinized cells and Ledig cells. The positive rate was significantly related to different histological types and the patients clinical endocrinal manifestation (P value were 0.01 and 0.041, respectively), but no statistically significant difference was found between the expression of hTRT and tumor histological grade or patients prognosis. Conclusions: In ovarian sex cord stromal tumors, the tumor component differentiating to luteinized stromal cell has high telomerase activity, and the telomerase activity may play an important role in patients endocrinal disorder.
出处
《癌症》
SCIE
CAS
CSCD
北大核心
2002年第1期87-90,共4页
Chinese Journal of Cancer
