摘要
目的分析子宫内膜异位症患者中γδ+T细胞和天然杀伤细胞(NK)功能的变化,以及探讨患者腹腔液对正常人γδ+T细胞和自然杀伤细胞(NK)功能的影响。方法采用免疫荧光细胞检测及细胞毒活性检测法,分析22例子宫内膜异位症患者外周血、腹腔液中γδ+T细胞及NK细胞的表型与功能。结果子宫内膜异位症(疾病组)患者外周血、腹腔液中CD56+细胞与正常对照组相比较无明显差异,而疾病组腹腔液中γδ+T细胞的百分率高于其外周血和正常对照组。疾病组腹腔液中的γδ+T细胞及NK细胞的功能低于外周血及正常对照组。疾病组的腹腔液对正常人外周血γδ+T细胞及NK细胞的功能具有负调节作用。结论子宫内膜异位症患者的γδ+T细胞及NK细胞的功能低下,处于被抑制状态,提示患者的腹腔液中,可能存在γδ+T细胞及NK细胞的功能抑制因子。
Aim To investigate the roles of γδ+T cell and NK cell activities in the development of endometriosis. Methods The phenotypes and activities of γδ+ T cells and NK cells in peripheral blood and peritoneal fluid of patients with endometriosis were studied by immunofluorescence assay and cytotoxicity assay. Result Percentage of γδ+ T cells in peritoneal fluid of the patients was higher than that in peripheral blood of the patients and in control. The activities of γδ+ T cells and NK cells in peripheral blood and peritoneal fluid of the patients were lower than those in peripheral blood and in control. The peritoneal fluid of the patients could decrease activities of γδ+T cells and NK cells in healthy.Conclusion Activities of γδ+ T cells and NK cells are low state, suggesting that there is suppressive factor directed at these cells in peritoneal fluid of the patients.
出处
《细胞与分子免疫学杂志》
CAS
CSCD
北大核心
2001年第6期540-541,共2页
Chinese Journal of Cellular and Molecular Immunology
