摘要
目的研究K562、Raji肿瘤细胞树突细胞(DC)融合瘤苗对脐血来源细胞因子诱导的杀伤(CIK)细胞/自然杀伤(NK)细胞杀伤作用的影响。方法诱导脐血单个核细胞成为DC、CIK/NK细胞,通过聚乙二醇将灭活K562和Raji肿瘤细胞株与DC融合形成K562-DC和RajiDC融合瘤苗。实验分为3组:DC融合瘤苗组、灭活肿瘤细胞加DC组、单纯DC组。以MTT法评价各组不同共孵育刺激对CIK/NK细胞杀伤活性的影响。结果使用不同抗原刺激的各组脐血来源CIK/NK细胞对特定肿瘤的杀伤有促进作用,但不具有特异性。在20∶1效靶比时,K562-DC和RajiDC融合瘤苗组对Raji细胞的杀伤率分别为(75.44±4.19)%和(81.33±4.18)%,RajiDC融合瘤苗组对Raji细胞的杀伤率显著高于K562-DC融合瘤苗组(P<0.05);灭活K562+DC和灭活Raji+DC组对Raji细胞的杀伤率分别为(73.12±4.22)%和(80.49±4.27)%,灭活Raji+DC组对Raji细胞的杀伤率显著高于灭活K562+DC组(P<0.01),各组对K562细胞的杀伤率比较差异无统计学意义(P>0.05)。不同效靶比CIK/NK细胞对不同种属的肿瘤细胞株的杀伤率比较,Raji细胞刺激的效果更强些。相同的肿瘤抗原刺激的或经灭活肿瘤细胞加DC刺激的CIK/NK细胞对不同种属的肿瘤细胞杀伤率差异无统计学意义。
Objectives To evaluate the effects of K562-dendritic cell (DC) and Raji-DC fusion vaccines on the cytotoxicity of cord blood (CB) derived cytokine-induced killer/natural killer(CIK/NK) cells.Methods DC and CIK /NK cells were derived from CB monouclear cells. CB-DC were fused with inactivated K562 or Raji cells by PEG to form K562 or Raji-DC fusion vaccine.The CIK /NK cells stimulated by different co-culture antigens were three groups: K562-DC or Raji-DC fusion vaccine group, inactivated K562 or Raji plus DC group,and CB-DC alone group. The cytotoxicity of CIK /NK cells stimulated by different co-culture antigens was measuced by MTT test. Results All the antigens used for stimulation could enhance the cytotoxicity of CB-CIK/NK cells,with no specificity difference. At 20∶1 effector-target ratio, the cytolytic activities of K562-DC and Raji-DC fusion vaccine groups against Raji cells were (75.44±4.19)% and ( 81.33±4.18)% respectively(P<0.05); and that of inactivated K562+DC and Raji+DC group against Raji cells were (73.12±4.22)% and(80.49±4.27)%, respectively(P<0.01).There was no significant difference in the cytotoxicity to K562 cells between the two fusion vaccine groups(P>0.05).The cytotoxicity of CB-CIK/NK cells immuned by Raji cells was higher than that by K562 cells. In CIK/NK cells co-stimulated by the same tumor antigen, there was no significant difference in the cytotoxicity between DC fusion vaccine group and inactivated cells plus DC group to different tumor cells. Conclusions The cytotoxicity of CB-CIK/NK cells to tumor cells was not specific. There was no significant difference in the cytotoxic activity of CB-CIK/NK cells between the DC fusion vaccine group and inactivated cells plus DC group.
出处
《中华血液学杂志》
CAS
CSCD
北大核心
2005年第5期269-272,共4页
Chinese Journal of Hematology
基金
广东省自然科学基金资助项目(031707)
卫生部临床重点基金资助项目(2004468)
关键词
树突细胞
肿瘤瘤苗
杀伤细胞
细胞因子
免疫细胞毒性
胎血
Dendritic cells
Tumor vaccines
Cytokine-induced killer cell
Cytotoxicity,immunity
Cord blood
