摘要
目的探讨氨基乙酰丙酸(ALA)对同种异体活化的外周血单个核细胞(PBMCs)的清除作用,摸索最佳ALA浓度和孵育时间。方法取正常人PBMCs进行单向淋巴细胞混合培养(MLC)5d后将其分为ALA组、光照组、ALA+光照组以及对照组。ALA组和ALA+光照组分别加入ALA终浓度为0.5、1.0、1.5、2.0、2.5mmol/L。将以上MLC细胞分别孵育2、4、6h后,光照组和ALA+光照组接受410nm光源照射1h。加入原去增殖的PBMCs孵育48h。使用MTT比色法进行检测,并计算ALAPDT后的单向MLC细胞对原去增殖PBMCs的杀伤率。通过杀伤率间接比较ALAPDT清除同种异体激活细胞的效果。结果ALA+光照组对刺激细胞的杀伤率明显低于光照组、ALA组和对照组,分别为(33.0±26.5)%比(87.1±2.2)%、(89.2±2.5)%、(90.3±1.9)%(P均<0.005)。2.0mmol/L、孵育4h组杀伤率最低(P均<0.005)。结论ALAPDT能够选择性清除活化的淋巴细胞,使针对同种异体、淋巴细胞介导的免疫排斥反应减轻甚至消失,从而减轻移植物抗宿主反应。
Objective To explore the effect of aminolevulinic acid - based photodynamic therapy（ALA- PDT） on alloreactived peripheral blood mononuelear cells（PBMCs）. Methods Human PBMCs from different healthy donor were reflected and mixed in the oneway mixed lymphocyte culture（MLC） for 5 days. The cells were harvested and aminolevulinic acld（ALA） were added into ALA group and ALA ± Light group with ultimate concentrations of 0.5 retool/L. 1.0 mnol/L, 1.5 mmol/L,2.0 mmol/L and 2.5 mmol/L. After cultured for 2 hours, 4 hours and 6 hours respectively in 37℃ 5 % carbon dioxide incubator, Light group and ALA ± Light group were irradiated by light of 410 nm wavelength for 1 hour. The MLC cells were treated with the former stimulator cells for 48 hour. The survival of stimulator cells were detected using MTT colorimetrie assay and the kill rate.s of treated cells were calculated. Results The kill rate of ALA ± Light group on stimulators was apparently lower than these of Light group, ALA group and control group, （33.0 ± 26.5） % vs （87.1 ± 2.2） %, （89.2 ± 2.5） %, （90.3 ± 1. 9） % （ All P 〈 0. 005）, respectively. The kill rate of 2.0 mmol/L. ALA- PDT after 4 hours incubation group were the lowest among all groups（P〈 0. 005）. Conclusions The data suggest the role of ALA - PDT in .selective dysfunction of alloreactived PBMCs, which is likely to be a treatment of disease that requires the selective dysfunction of alloreactived PBMCs, and probably relieve the araf- versus - host disease.
出处
《实用儿科临床杂志》
CSCD
北大核心
2005年第12期1176-1178,共3页
Journal of Applied Clinical Pediatrics
基金
国家自然科学基金项目资助(30472007)
陕西省科技研究发展计划项目资助[2004k12-G6(2)]
关键词
治疗学
光动力疗法
白细胞
淋巴细胞
光敏剂
氨基乙酰丙酸
therapeutics, pholodynamic therapy
leukocyte
lymphocyte
photosensitizing agent
aminolevulinic acid
