摘要
目的 建立慢性排斥反应的简化动物模型，观察移植体动脉硬化发展规律。方法 采用病理图像分析及电镜观察移植后３、７、１４、２０、３０、６０ ｄ移植动脉硬化情况，结合 ＰＣＮＡ及 Ｃ－ｍｙｃ免疫组化了解细胞增殖与原癌基因表达的关系。结果①术后１４ ｄ内膜层明显增生，术后 ６０ ｄ持续增厚，细胞数术后 ３０ ｄ达高峰，术后 ２０ ｄ内膜层出现大量合成型平滑肌细胞；②术后 １４ ｄ中层细胞变性坏死，向内层迁移，α－ａｃｔｉｎ阳性细胞数下降，而内膜层α－ａｃｔｉｎ阳性细胞数却迅速升高，两者呈显著负相关；③术后３ ｄ外膜大量炎性细胞浸润。结论①移植动脉硬化具有内膜增生，中层细胞坏死、迁移、炎细胞浸润三大特点；②内膜增生在术后早期以细胞增生为主，后期则为细胞外基质增生，且伴随平滑肌形态改变；③ＰＣＮＡ表达反映出细胞增殖进程，而Ｃ－ｍｙｃ还可能与非增殖因素有关。
Objective To establish the experimental animal model of chronic rejection and observe the development of arteriosclerosis in the transplant. Methods The pathological changes of transplanted aorta at 3, 7, 14,20, 30 and 60 days after transplantation were observed by electron microscopy and computer image analysis. PCNA and C-myc were detected by immunohistochemical method. Results ①The intime thickening appeared at 14 day after operation, the intima hyperplasia developed persistently until 60 day and the cell numbers showed a peak at 30th day after operation. A great many synthetic smooth muscle cell in the intima membrane appeared at 20 day after operation. ②The cell necrosis in the media and the cell migrated from the media to initma were observed, the number of α-actin positive cell decreased in the media, while increased in the intima at 14 day after operation. A significant negative correlation existed between them. ③The exprssion of PCNA showed a peak at 20 day after opera- tion. The expression of C-myc and PCNA showed a negative correlation. ④Inflammatory cells infitrated in the adventitia at 3 day after operation. Conclusion ①Three characteristics including intima hyperplasia, media necrosis and cell migration and inflammatory cell infiltation are demonstrat- ed in this model. ②In early phase the intima appears mainly in cell proliferation but extracellular matrix production deposition in later phase and the smooth muscle cell may undergo a phenotypic change during their proliferation process. ③The expression of PCNA shows significant correlation with cell proliferation, the expression of C-myc may be associated with some non-proliferative factors.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2001年第8期900-903,共4页
Journal of Third Military Medical University
